Isolation of neural progenitor cells from the human adult subventricular zone based on expression of the cell surface marker CD271

TY - JOUR

T1 - Isolation of neural progenitor cells from the human adult subventricular zone based on expression of the cell surface marker CD271

AU - van Strien, Miriam E

AU - Sluijs, Jacqueline A

AU - Reynolds, Brent A

AU - Steindler, Dennis A

AU - Aronica, Eleonora

AU - Hol, Elly M

PY - 2014/4

Y1 - 2014/4

N2 - Neural progenitor cells (NPCs) in the subventricular zone (SVZ) hold promise for future therapy for neurodegenerative disorders, because the stimulation of adult neurogenesis could potentially restore the function of degenerating neurons and glia. To obtain more knowledge on these NPCs, we developed a method to specifically isolate NPCs from postmortem adult human brains based on the expression of the specific human adult neural stem/progenitor cell marker glial fibrillary acidic protein δ (GFAPδ). An extensive immunophenotyping analysis for cell surface markers resulted in the observation that CD271 was limited to the SVZ-derived GFAPδ-positive cells. CD271(+) cells developed into neurospheres and could be differentiated into astrocytes, neurons, and oligodendrocytes. We are the first to show that a pure population of NPCs can be isolated from the adult human SVZ, which is highly instrumental for developing future therapies based on stimulating endogenous SVZ neurogenesis.

AB - Neural progenitor cells (NPCs) in the subventricular zone (SVZ) hold promise for future therapy for neurodegenerative disorders, because the stimulation of adult neurogenesis could potentially restore the function of degenerating neurons and glia. To obtain more knowledge on these NPCs, we developed a method to specifically isolate NPCs from postmortem adult human brains based on the expression of the specific human adult neural stem/progenitor cell marker glial fibrillary acidic protein δ (GFAPδ). An extensive immunophenotyping analysis for cell surface markers resulted in the observation that CD271 was limited to the SVZ-derived GFAPδ-positive cells. CD271(+) cells developed into neurospheres and could be differentiated into astrocytes, neurons, and oligodendrocytes. We are the first to show that a pure population of NPCs can be isolated from the adult human SVZ, which is highly instrumental for developing future therapies based on stimulating endogenous SVZ neurogenesis.

KW - Adult

KW - Antigens, Differentiation

KW - Astrocytes

KW - Brain

KW - Cell Differentiation

KW - Cell Separation

KW - Female

KW - Gene Expression

KW - Glial Fibrillary Acidic Protein

KW - Humans

KW - Male

KW - Nerve Tissue Proteins

KW - Neural Stem Cells

KW - Neurogenesis

KW - Neurons

KW - Oligodendroglia

KW - Receptors, Nerve Growth Factor

U2 - 10.5966/sctm.2013-0038

DO - 10.5966/sctm.2013-0038

M3 - Article

C2 - 24604282

SN - 2157-6564

VL - 3

SP - 470

EP - 480

JO - Stem cells translational medicine

JF - Stem cells translational medicine

IS - 4

ER -