Live imaging of cell division in 3D stem-cell organoid cultures

Ana C F Bolhaqueiro, Richard H van Jaarsveld, Bas Ponsioen, René M Overmeer, Hugo J Snippert, Geert J P L Kops

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.

Original languageEnglish
Pages (from-to)91-106
Number of pages16
JournalMethods in Cell Biology
Volume145
DOIs
Publication statusPublished - 2018

Keywords

  • Animals
  • Cell Culture Techniques/methods
  • Cell Division/physiology
  • Humans
  • Microscopy/methods
  • Organoids/physiology
  • Stem Cells/physiology

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