Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Jiahui Wu; Sara Zaccara; Deepak Khuperkar; Hyaeyeong Kim; Marvin E Tanenbaum; Samie R Jaffrey

doi:10.1038/s41592-019-0531-7

Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Jiahui Wu, Sara Zaccara, Deepak Khuperkar, Hyaeyeong Kim, Marvin E Tanenbaum, Samie R Jaffrey

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

71 Citations (Scopus)

Abstract

Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.

Original language	English
Pages (from-to)	862-865
Number of pages	4
Journal	Nature Methods
Volume	16
Issue number	9
DOIs	https://doi.org/10.1038/s41592-019-0531-7
Publication status	Published - Sept 2019

Keywords

Aptamers, Nucleotide/genetics
Bacterial Proteins/genetics
Fluorescence
Fluorescent Dyes/chemistry
HEK293 Cells
Humans
Luminescent Proteins/genetics
Microscopy, Fluorescence/methods
Molecular Imaging/methods
RNA, Messenger/analysis

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10.1038/s41592-019-0531-7

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title = "Live imaging of mRNA using RNA-stabilized fluorogenic proteins",

abstract = "Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.",

keywords = "Aptamers, Nucleotide/genetics, Bacterial Proteins/genetics, Fluorescence, Fluorescent Dyes/chemistry, HEK293 Cells, Humans, Luminescent Proteins/genetics, Microscopy, Fluorescence/methods, Molecular Imaging/methods, RNA, Messenger/analysis",

author = "Jiahui Wu and Sara Zaccara and Deepak Khuperkar and Hyaeyeong Kim and Tanenbaum, {Marvin E} and Jaffrey, {Samie R}",

year = "2019",

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doi = "10.1038/s41592-019-0531-7",

language = "English",

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T1 - Live imaging of mRNA using RNA-stabilized fluorogenic proteins

AU - Wu, Jiahui

AU - Zaccara, Sara

AU - Khuperkar, Deepak

AU - Kim, Hyaeyeong

AU - Tanenbaum, Marvin E

AU - Jaffrey, Samie R

PY - 2019/9

Y1 - 2019/9

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AB - Fluorogenic RNA aptamers bind and activate the fluorescence of otherwise nonfluorescent dyes. However, fluorogenic aptamers are limited by the small number of fluorogenic dyes suitable for use in live cells. In this communication, fluorogenic proteins whose fluorescence is activated by RNA aptamers are described. Fluorogenic proteins are highly unstable until they bind RNA aptamers inserted into messenger RNAs, resulting in fluorescent RNA-protein complexes that enable live imaging of mRNA in living cells.

KW - Aptamers, Nucleotide/genetics

KW - Bacterial Proteins/genetics

KW - Fluorescence

KW - Fluorescent Dyes/chemistry

KW - HEK293 Cells

KW - Humans

KW - Luminescent Proteins/genetics

KW - Microscopy, Fluorescence/methods

KW - Molecular Imaging/methods

KW - RNA, Messenger/analysis

U2 - 10.1038/s41592-019-0531-7

DO - 10.1038/s41592-019-0531-7

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VL - 16

SP - 862

EP - 865

JO - Nature Methods

JF - Nature Methods

IS - 9

ER -

Live imaging of mRNA using RNA-stabilized fluorogenic proteins

Abstract

Keywords

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