TY - JOUR
T1 - Low-cost, rapid and reliable DNA extraction from mammalian organoids using a C. elegans toolbox
AU - Mars, Jonas
AU - Darmasaputra, Gabriella S
AU - Galli, Matilde
AU - Korswagen, Hendrik C
N1 - Copyright: © 2024 by the authors.
PY - 2024
Y1 - 2024
N2 - High-quality DNA extraction from organoids is an important step in molecular genetics research. Here, we show that a lysis buffer from the field of Caenorhabditis elegans research, called Single Worm Lysis Buffer (SWLB), is a low-cost, yet reliable method for DNA extraction from mammalian organoids. SWLB is superior in terms of price, storage, hands-on time and sustainability compared to current standardized DNA extraction protocols, while equally effective. This work indicates that it is useful to compare methods from different model systems, such as mammalian organoids and invertebrate nematodes, to find useful alternatives for research methodologies.
AB - High-quality DNA extraction from organoids is an important step in molecular genetics research. Here, we show that a lysis buffer from the field of Caenorhabditis elegans research, called Single Worm Lysis Buffer (SWLB), is a low-cost, yet reliable method for DNA extraction from mammalian organoids. SWLB is superior in terms of price, storage, hands-on time and sustainability compared to current standardized DNA extraction protocols, while equally effective. This work indicates that it is useful to compare methods from different model systems, such as mammalian organoids and invertebrate nematodes, to find useful alternatives for research methodologies.
U2 - 10.17912/micropub.biology.001240
DO - 10.17912/micropub.biology.001240
M3 - Article
C2 - 38983900
SN - 2578-9430
VL - 2024
JO - microPublication biology
JF - microPublication biology
ER -