M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue

Ivone M Martins, Alexandre Lima, Wim de Graaff, Joana S Cristóvão, Niek Brosens, Eleonora Aronica, Leon D Kluskens, Cláudio M Gomes, Joana Azeredo, Helmut W Kessels

Research output: Contribution to journal/periodicalArticleScientificpeer-review

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Abstract

Oligomeric clusters of amyloid-β (Aβ) are one of the major biomarkers for Alzheimer's disease (AD). However, proficient methods to detect Aβ-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying Aβ-derived peptides on their surface preferentially interact with Aβ-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized Aβ-aggregates in hippocampus at an early age, prior to the occurrence of Aβ-plaques. Similarly, the bacteriophages reveal the presence of such small Aβ-aggregates in post-mortem hippocampus tissue of AD-patients. These results advocate bacteriophages displaying Aβ-peptides as a convenient and low-cost tool to identify Aβ-oligomers in post-mortem brain tissue of AD-model mice and AD-patients.

Original languageEnglish
Pages (from-to)134
JournalCommunications Biology
Volume7
Issue number1
DOIs
Publication statusPublished - 27 Jan 2024

Keywords

  • Humans
  • Mice
  • Animals
  • Amyloid beta-Peptides/metabolism
  • Amyloid beta-Protein Precursor/metabolism
  • Bacteriophage M13/metabolism
  • Alzheimer Disease
  • Mice, Transgenic
  • Brain/metabolism

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