Abstract
Oligomeric clusters of amyloid-β (Aβ) are one of the major biomarkers for Alzheimer's disease (AD). However, proficient methods to detect Aβ-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying Aβ-derived peptides on their surface preferentially interact with Aβ-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized Aβ-aggregates in hippocampus at an early age, prior to the occurrence of Aβ-plaques. Similarly, the bacteriophages reveal the presence of such small Aβ-aggregates in post-mortem hippocampus tissue of AD-patients. These results advocate bacteriophages displaying Aβ-peptides as a convenient and low-cost tool to identify Aβ-oligomers in post-mortem brain tissue of AD-model mice and AD-patients.
Original language | English |
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Pages (from-to) | 134 |
Journal | Communications Biology |
Volume | 7 |
Issue number | 1 |
DOIs | |
Publication status | Published - 27 Jan 2024 |
Keywords
- Humans
- Mice
- Animals
- Amyloid beta-Peptides/metabolism
- Amyloid beta-Protein Precursor/metabolism
- Bacteriophage M13/metabolism
- Alzheimer Disease
- Mice, Transgenic
- Brain/metabolism