Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

E. Berezikov; G. van Tetering; M. Verheul; J. van de Belt; L.W. van Laake; J. Vos; R. Verloop; M.L. van de Wetering; V. Guryev; S. Takada; A.J. van Zonneveld; H. Mano; R. Plasterk; E. Cuppen

doi:10.1101/gr.5159906

Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

E. Berezikov, G. van Tetering, M. Verheul, J. van de Belt, L.W. van Laake, J. Vos, R. Verloop, M.L. van de Wetering, V. Guryev, S. Takada, A.J. van Zonneveld, H. Mano, R. Plasterk, E. Cuppen

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

240 Citations (Scopus)

Abstract

MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently > 400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.

Original language	English
Pages (from-to)	1289-1298
Number of pages	10
Journal	Genome Research
Volume	16
Issue number	10
DOIs	https://doi.org/10.1101/gr.5159906
Publication status	Published - 2006

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Berezikov, E., van Tetering, G., Verheul, M., van de Belt, J., van Laake, L. W., Vos, J., Verloop, R., van de Wetering, M. L., Guryev, V., Takada, S., van Zonneveld, A. J., Mano, H., Plasterk, R., & Cuppen, E. (2006). Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis. Genome Research, 16(10), 1289-1298. https://doi.org/10.1101/gr.5159906

@article{53afec39cd394876a6f21e1681e5eb94,

title = "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis",

abstract = "MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently > 400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.",

author = "E. Berezikov and {van Tetering}, G. and M. Verheul and {van de Belt}, J. and {van Laake}, L.W. and J. Vos and R. Verloop and {van de Wetering}, M.L. and V. Guryev and S. Takada and {van Zonneveld}, A.J. and H. Mano and R. Plasterk and E. Cuppen",

note = "Reporting year: 2006 Metis note: 16954537",

year = "2006",

doi = "10.1101/gr.5159906",

language = "English",

volume = "16",

pages = "1289--1298",

journal = "Genome Research",

issn = "1088-9051",

publisher = "Cold Spring Harbor Laboratory Press",

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Berezikov, E, van Tetering, G, Verheul, M, van de Belt, J, van Laake, LW, Vos, J, Verloop, R, van de Wetering, ML, Guryev, V, Takada, S, van Zonneveld, AJ, Mano, H, Plasterk, R & Cuppen, E 2006, 'Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis', Genome Research, vol. 16, no. 10, pp. 1289-1298. https://doi.org/10.1101/gr.5159906

TY - JOUR

T1 - Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

AU - Berezikov, E.

AU - van Tetering, G.

AU - Verheul, M.

AU - van de Belt, J.

AU - van Laake, L.W.

AU - Vos, J.

AU - Verloop, R.

AU - van de Wetering, M.L.

AU - Guryev, V.

AU - Takada, S.

AU - van Zonneveld, A.J.

AU - Mano, H.

AU - Plasterk, R.

AU - Cuppen, E.

N1 - Reporting year: 2006 Metis note: 16954537

PY - 2006

Y1 - 2006

N2 - MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently > 400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.

AB - MicroRNAs are 20- to 23-nucleotide RNA molecules that can regulate gene expression. Currently > 400 microRNAs have been experimentally identified in mammalian genomes, whereas estimates go up to 1000 and beyond. Here we show that many more mammalian microRNAs exist. We discovered novel microRNA candidates using two approaches: testing of computationally predicted microRNAs by a modified microarray-based detection system, and cloning and sequencing of large numbers of small RNAs from different human and mouse tissues. Together these efforts experimentally identified 348 novel mouse and 81 novel human microRNA candidate genes. Most novel microRNAs candidates are not conserved beyond mammals, and ~10% are taxon-specific. Our analyses indicate that the entire microRNA repertoire is not remotely exhausted.

U2 - 10.1101/gr.5159906

DO - 10.1101/gr.5159906

M3 - Article

SN - 1088-9051

VL - 16

SP - 1289

EP - 1298

JO - Genome Research

JF - Genome Research

IS - 10

ER -

Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis

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