Abstract
Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds.
Original language | English |
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Pages (from-to) | 299-312 |
Journal | Journal of Industrial Microbiology and Biotechnology |
Volume | 43 |
Issue number | 2-3 |
DOIs | |
Publication status | Published - Mar 2016 |
Keywords
- Metabolomics
- NMR
- Antibiotic production
- Streptomyces
- Antimicrobial drug resistance
- national