Metabolic profiling as a tool for prioritizing antimicrobial compounds

Changsheng Wu, Young Hae Choi, Gilles P. van Wezel

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Abstract

Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds.
Original languageEnglish
Pages (from-to)299-312
JournalJournal of Industrial Microbiology and Biotechnology
Volume43
Issue number2-3
DOIs
Publication statusPublished - Mar 2016

Keywords

  • Metabolomics
  • NMR
  • Antibiotic production
  • Streptomyces
  • Antimicrobial drug resistance
  • national

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