Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds.
|Journal||Journal of Industrial Microbiology and Biotechnology|
|Publication status||Published - Mar 2016|
- Antibiotic production
- Antimicrobial drug resistance
Wu, C., Choi, Y. H., & van Wezel, G. P. (2016). Metabolic profiling as a tool for prioritizing antimicrobial compounds. Journal of Industrial Microbiology and Biotechnology, 43(2-3), 299-312. https://doi.org/10.1007/s10295-015-1666-x