Metabolic profiling as a tool for prioritizing antimicrobial compounds

TY - JOUR

T1 - Metabolic profiling as a tool for prioritizing antimicrobial compounds

AU - Wu, Changsheng

AU - Choi, Young Hae

AU - van Wezel, Gilles P.

N1 - 6080, ME; Data archiving: property of and archived at Leiden University

PY - 2016/3

Y1 - 2016/3

N2 - Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds.

AB - Metabolomics is an analytical technique that allows scientists to globally profile low molecular weight metabolites between samples in a medium- or high throughput environment. Different biological samples are statistically analyzed and correlated to a bioactivity of interest, highlighting differentially produced compounds as potential biomarkers. Here, we review NMR- and MS based metabolomics as technologies to facilitate the identification of novel antimicrobial natural products from microbial sources. Approaches to elicit the production of poorly expressed (cryptic) molecules are thereby a key to allow statistical analysis of samples to identify bioactive markers, while connection of compounds to their biosynthetic gene cluster is a determining step in elucidating the biosynthetic pathway and allows downstream process optimization and upscaling. The review focuses on approaches built around NMR-based metabolomics, which enables efficient dereplication and guided fractionation of (antimicrobial) compounds.

KW - Metabolomics

KW - NMR

KW - Antibiotic production

KW - Streptomyces

KW - Antimicrobial drug resistance

KW - national

U2 - 10.1007/s10295-015-1666-x

DO - 10.1007/s10295-015-1666-x

M3 - Article

SN - 1367-5435

VL - 43

SP - 299

EP - 312

JO - Journal of Industrial Microbiology and Biotechnology

JF - Journal of Industrial Microbiology and Biotechnology

IS - 2-3

ER -