Microbial and volatile profiling of soils suppressive to Fusarium culmorum of wheat

TY - JOUR

T1 - Microbial and volatile profiling of soils suppressive to Fusarium culmorum of wheat

AU - Ossowicki, Adam

AU - Tracanna, Vittorio

AU - Petrus, M.L.C.

AU - van Wezel, Gilles

AU - Raaijmakers, J.M.

AU - Medema, Marnix H

AU - Garbeva, Paolina

N1 - 6904, ME; Data Archived at Dryad

PY - 2020/2/26

Y1 - 2020/2/26

N2 - In disease-suppressive soils, microbiota protect plants from root infections. Bacterial members of this microbiota have been shown to produce specific molecules that mediate this phenotype. To date, however, studies have focused on individual suppressive soils and the degree of natural variability of soil suppressiveness remains unclear. Here, we screened a large collection of field soils for suppressiveness to Fusarium culmorum using wheat ( Triticum aestivum) as a model host plant. A high variation of disease suppressiveness was observed, with 14% showing a clear suppressive phenotype. The microbiological basis of suppressiveness to F. culmorum was confirmed by gamma sterilization and soil transplantation. Amplicon sequencing revealed diverse bacterial taxonomic compositions and no specific taxa were found exclusively enriched in all suppressive soils. Nonetheless, co-occurrence network analysis revealed that two suppressive soils shared an overrepresented bacterial guild dominated by various Acidobacteria. In addition, our study revealed that volatile emission may contribute to suppression, but not for all suppressive soils. Our study raises new questions regarding the possible mechanistic variability of disease-suppressive phenotypes across physico-chemically different soils. Accordingly, we anticipate that larger-scale soil profiling, along with functional studies, will enable a deeper understanding of disease-suppressive microbiomes.

AB - In disease-suppressive soils, microbiota protect plants from root infections. Bacterial members of this microbiota have been shown to produce specific molecules that mediate this phenotype. To date, however, studies have focused on individual suppressive soils and the degree of natural variability of soil suppressiveness remains unclear. Here, we screened a large collection of field soils for suppressiveness to Fusarium culmorum using wheat ( Triticum aestivum) as a model host plant. A high variation of disease suppressiveness was observed, with 14% showing a clear suppressive phenotype. The microbiological basis of suppressiveness to F. culmorum was confirmed by gamma sterilization and soil transplantation. Amplicon sequencing revealed diverse bacterial taxonomic compositions and no specific taxa were found exclusively enriched in all suppressive soils. Nonetheless, co-occurrence network analysis revealed that two suppressive soils shared an overrepresented bacterial guild dominated by various Acidobacteria. In addition, our study revealed that volatile emission may contribute to suppression, but not for all suppressive soils. Our study raises new questions regarding the possible mechanistic variability of disease-suppressive phenotypes across physico-chemically different soils. Accordingly, we anticipate that larger-scale soil profiling, along with functional studies, will enable a deeper understanding of disease-suppressive microbiomes.

KW - national

KW - Plan_S-Compliant_NO

KW - Microbiota

KW - Plant Roots/microbiology

KW - Soil/chemistry

KW - Triticum/microbiology

KW - Soil Microbiology

KW - Fusarium/physiology

KW - Fusarium culmorum

KW - Soil volatiles

KW - Soil suppressiveness

KW - Rhizosphere microbiome

UR - https://dx.doi.org/10.5061/dryad.g884q70

U2 - 10.1098/rspb.2019.2527

DO - 10.1098/rspb.2019.2527

M3 - Article

C2 - 32070256

SN - 0962-8452

VL - 287

SP - 20192527

JO - Proceedings of the Royal Society B-Biological Sciences

JF - Proceedings of the Royal Society B-Biological Sciences

IS - 1921

M1 - 20192527.

ER -