Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated

Marjolein A M Sneeboer; Gijsje J L J Snijders; Woutje M Berdowski; Alba Fernández-Andreu; Hans C van Mierlo; Amber Berdenis van Berlekom; Manja Litjens; René S Kahn; Elly M Hol; Lot D de Witte

doi:10.1038/s41398-019-0490-x

Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated

Marjolein A M Sneeboer, Gijsje J L J Snijders, Woutje M Berdowski, Alba Fernández-Andreu, , Hans C van Mierlo, Amber Berdenis van Berlekom, Manja Litjens, René S Kahn, Elly M Hol, Lot D de Witte

Netherlands Institute for Neuroscience (NIN)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

45 Citations (Scopus)

160 Downloads (Pure)

Abstract

Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.

Original language	English
Pages (from-to)	153
Journal	Translational Psychiatry
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41398-019-0490-x
Publication status	Published - Apr 2019

Access to Document

10.1038/s41398-019-0490-x

Sneeboer2019Final published version, 1.72 MB

Cite this

Sneeboer, M. A. M., Snijders, G. J. L. J., Berdowski, W. M., Fernández-Andreu, A., van Mierlo, H. C., Berdenis van Berlekom, A., Litjens, M., Kahn, R. S., & Hol, E. M., & de Witte, L. D. (2019). Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated. Translational Psychiatry, 9(1), 153. https://doi.org/10.1038/s41398-019-0490-x

@article{df8c13c9ba9948de989db673f9a51e88,

title = "Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated",

abstract = "Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.",

author = "Sneeboer, {Marjolein A M} and Snijders, {Gijsje J L J} and Berdowski, {Woutje M} and Alba Fern{\'a}ndez-Andreu and {van Mierlo}, {Hans C} and {Berdenis van Berlekom}, Amber and Manja Litjens and Kahn, {Ren{\'e} S} and Hol, {Elly M} and {de Witte}, {Lot D}",

year = "2019",

month = apr,

doi = "10.1038/s41398-019-0490-x",

language = "English",

volume = "9",

pages = "153",

journal = "Translational Psychiatry",

issn = "2158-3188",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated

AU - Sneeboer, Marjolein A M

AU - Snijders, Gijsje J L J

AU - Berdowski, Woutje M

AU - Fernández-Andreu, Alba

AU - van Mierlo, Hans C

AU - Berdenis van Berlekom, Amber

AU - Litjens, Manja

AU - Kahn, René S

AU - Hol, Elly M

AU - de Witte, Lot D

PY - 2019/4

Y1 - 2019/4

N2 - Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.

AB - Genetic, epidemiological, and biomarker studies suggest that the immune system is involved in the pathogenesis of bipolar disorder (BD). It has therefore been hypothesized that immune activation of microglia, the resident immune cells of the brain, is associated with the disease. Only a few studies have addressed the involvement of microglia in BD so far and a more detailed immune profiling of microglial activation is lacking. Here, we applied a multi-level approach to determine the activation state of microglia in BD post-mortem brain tissue. We did not find differences in microglial density, and mRNA expression of microglial markers in the medial frontal gyrus (MFG) of patients with BD. Furthermore, we performed in-depth characterization of human primary microglia isolated from fresh brain tissue of the MFG, superior temporal gyrus (STG), and thalamus (THA). Similarly, these ex vivo isolated microglia did not show elevated expression of inflammatory markers. Finally, challenging the isolated microglia with LPS did not result in an increased immune response in patients with BD compared to controls. In conclusion, our study shows that microglia in post-mortem brain tissue of patients with BD are not immune activated.

U2 - 10.1038/s41398-019-0490-x

DO - 10.1038/s41398-019-0490-x

M3 - Article

C2 - 31127084

SN - 2158-3188

VL - 9

SP - 153

JO - Translational Psychiatry

JF - Translational Psychiatry

IS - 1

ER -

Microglia in post-mortem brain tissue of patients with bipolar disorder are not immune activated

Abstract

Access to Document

Fingerprint

Cite this