A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of microheterogeneity typical of sequence clusters detected in environmental clone libraries. [KEYWORDS: Ribosomal-rna genes; polymerase chain-reaction; chimeric molecules; dna amplification; escherichia-coli heteroduplexes; populations; coamplification; consequence; diversity]
Original languageEnglish
Pages (from-to)469-472
JournalApplied and Environmental Microbiology
Issue number1
Publication statusPublished - 2001

ID: 235170