Modeling Human Digestive Diseases With CRISPR-Cas9-Modified Organoids

TY - JOUR

T1 - Modeling Human Digestive Diseases With CRISPR-Cas9-Modified Organoids

AU - Fujii, Masayuki

AU - Clevers, Hans

AU - Sato, Toshiro

N1 - Copyright © 2018 AGA Institute. Published by Elsevier Inc. All rights reserved.

PY - 2018/11/23

Y1 - 2018/11/23

N2 - Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients' tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.

AB - Insights into the stem cell niche have allowed researchers to cultivate adult tissue stem cells as organoids that display structural and phenotypic features of healthy and diseased epithelial tissues. Organoids derived from patients' tissues are used as models of disease and to test drugs. CRISPR-Cas9 technology can be used to genetically engineer organoids for studies of monogenic diseases and cancer. We review the derivation of organoids from human gastrointestinal tissues and how CRISPR-Cas9 technology can be used to study these organoids. We discuss burgeoning technologies that are broadening our understanding of diseases of the digestive system.

U2 - 10.1053/j.gastro.2018.11.048

DO - 10.1053/j.gastro.2018.11.048

M3 - Article

C2 - 30476497

SN - 0016-5085

JO - Gastroenterology

JF - Gastroenterology

ER -