TY - JOUR
T1 - Molecular characterization of cyanobacterial diversity in a shallow eutrophic lake
AU - Zwart, G.
AU - Kamst-van Agterveld, M.P.
AU - Van der Werff-Staverman, I.
AU - Hagen, F.
AU - Hoogveld, H.L.
AU - Gons, H.J.
N1 - Reporting year: 2005
Metis note: 3380; CL; MWE; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2005/Zwart_ea_3380.pdf
PY - 2005
Y1 - 2005
N2 - We have studied the diversity of pelagic cyanobacteria in Lake Loosdrecht, the Netherlands, through recovery and analysis of small subunit ribosomal RNA gene sequences from lake samples and cyanobacterial isolates. We used an adapted protocol for specific amplification of cyanobacterial rDNA for denaturing gradient gel electrophoresis (DGGE) analysis. This protocol enabled direct comparison of cyanobacterial community profiles with overall bacterial profiles. The theoretical amplification specificity of the primers was supported by sequence analysis of DNA from excised DGGE bands. Sequences recovered from these bands, in addition to sequences obtained by polymerase chain reaction (PCR) and cloning from lake DNA as well as from cyanobacterial isolates from the lake, revealed a diverse consortium of cyanobacteria, among which are representatives of the genera Aphanizomenon, Planktothrix, Microcystis and Synechococcus. One numerically important and persistent cyanobacterium in the lake, Prochlorothrix hollandica, appeared to co-occur with an unknown but related species. However, the lake is dominated by filamentous species that originally have been termed 'Oscillatoria limnetica-like'. We show that this is a group of several related cyanobacteria, co-occurring in the lake, which belong to the Limnothrix/Pseudanabaena group. The available variation among the coexisting strains of this group can explain the persistent dominance of the group under severe viral pressure.
AB - We have studied the diversity of pelagic cyanobacteria in Lake Loosdrecht, the Netherlands, through recovery and analysis of small subunit ribosomal RNA gene sequences from lake samples and cyanobacterial isolates. We used an adapted protocol for specific amplification of cyanobacterial rDNA for denaturing gradient gel electrophoresis (DGGE) analysis. This protocol enabled direct comparison of cyanobacterial community profiles with overall bacterial profiles. The theoretical amplification specificity of the primers was supported by sequence analysis of DNA from excised DGGE bands. Sequences recovered from these bands, in addition to sequences obtained by polymerase chain reaction (PCR) and cloning from lake DNA as well as from cyanobacterial isolates from the lake, revealed a diverse consortium of cyanobacteria, among which are representatives of the genera Aphanizomenon, Planktothrix, Microcystis and Synechococcus. One numerically important and persistent cyanobacterium in the lake, Prochlorothrix hollandica, appeared to co-occur with an unknown but related species. However, the lake is dominated by filamentous species that originally have been termed 'Oscillatoria limnetica-like'. We show that this is a group of several related cyanobacteria, co-occurring in the lake, which belong to the Limnothrix/Pseudanabaena group. The available variation among the coexisting strains of this group can explain the persistent dominance of the group under severe viral pressure.
U2 - 10.1111/j.1462-2920.2005.00715.x
DO - 10.1111/j.1462-2920.2005.00715.x
M3 - Article
SN - 1462-2912
VL - 7
SP - 365
EP - 377
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 3
ER -