Molecular detection and quantification of the Striga seedbank in agricultural soils

Getahun Mitiku Benti, Dominika Rybka, P.J.A. Klein Gunnewiek, Taye Tessema, J.M. Raaijmakers, Desalegn Etalo* (Corresponding author)

*Corresponding author for this work

Research output: Contribution to journal/periodicalArticleScientificpeer-review

16 Downloads (Pure)


Striga hermonthica (Del.) Benth is a devastating parasitic weed in Sub-Saharan Africa (SSA) and its soil seedbank is the major factor contributing to its prevalence and persistence. To date, there is a little information on the Striga seedbank density in agricultural fields in SSA due to the lack of reliable detection and quantification methods. We developed a high-throughput method that combines density- and size-based separation techniques with quantitative polymerase chain reaction (qPCR)-based detection of Striga seeds in soil. The method was optimised and validated by introducing increasing numbers of Striga seeds in two physicochemically different Striga-free agricultural soils. The results showed that as little as one seed of S. hermonthica per 150 g of soil could be detected. This technique was subsequently tested on soil samples of 48 sorghum fields from different agro-ecological zones in Ethiopia to map the geospatial distribution of the Striga seedbank along a trajectory of more than 1500 km. Considerable variation in Striga seed densities was observed. Striga seeds were detectable in 75% of the field soils with densities up to 86 seeds per 150 g of soil. The Striga seed density in soil and the number of emerged Striga plants in the field showed a non-linear relationship. In conclusion, the method developed allows for accurate mapping of the Striga seedbank in physicochemically diverse SSA field soils and can be used to assess the impact of management strategies on Striga seedbank dynamics.
Original languageEnglish
Pages (from-to)181-191
Number of pages11
JournalWeed Research
Issue number3
Early online date2022
Publication statusPublished - 2022


  • mapping
  • qPCR
  • sorghum field
  • Striga seed density
  • sub-Sahara Africa
  • weed seed


Dive into the research topics of 'Molecular detection and quantification of the Striga seedbank in agricultural soils'. Together they form a unique fingerprint.

Cite this