TY - JOUR
T1 - Molecular identification tools for sibling species of Scedosporium and Pseudallescheria
AU - Lackner, M.
AU - Klaassen, C.H.
AU - Meis, J.F.
AU - Gerrits van den Ende, A.H.G.
AU - de Hoog, G.S.
N1 - Reporting year: 2012
PY - 2012
Y1 - 2012
N2 - The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 ß-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was less detailed. ITS was sufficient for the differentiation of most haplotypes of clinically relevant species (P. apiosperma, P. boydii, S. aurantiacum, S. dehoogii, and S. prolificans) and of environmental species (P. minutispora and Lophotrichus fimeti) when Restriction Fragment Length Polymorphism (RFLP) were applied. For the identification of P. apiosperma and P. boydii species-specific BT2 primers were needed. Pseudallescheria fusoidea, P. ellipsoidea and P. angusta remained difficult to distinguish from P. boydii.
Keywords : Internal Transcribed Spacer, β-tubulin, RFLP, AFLP, species-specific primers, Pseudallescheria, Scedosporium
AB - The aim of this study was to develop molecular identification tools for currently recognized species of Pseudallescheria and Scedosporium through the use of species-specific primers and RFLP, so as to enhance rapid differentiation of clinically relevant species. The variability of species was established in a set of 681 Internal Transcribed Spacer (ITS) and 349 ß-tubulin (BT2) sequences. Amplified Fragment Length Polymorphism profile clustering matched with BT2 results, whereas ITS grouping was less detailed. ITS was sufficient for the differentiation of most haplotypes of clinically relevant species (P. apiosperma, P. boydii, S. aurantiacum, S. dehoogii, and S. prolificans) and of environmental species (P. minutispora and Lophotrichus fimeti) when Restriction Fragment Length Polymorphism (RFLP) were applied. For the identification of P. apiosperma and P. boydii species-specific BT2 primers were needed. Pseudallescheria fusoidea, P. ellipsoidea and P. angusta remained difficult to distinguish from P. boydii.
Keywords : Internal Transcribed Spacer, β-tubulin, RFLP, AFLP, species-specific primers, Pseudallescheria, Scedosporium
U2 - 10.3109/13693786.2011.618939
DO - 10.3109/13693786.2011.618939
M3 - Article
SN - 1369-3786
VL - 50
SP - 497
EP - 508
JO - Medical Mycology
JF - Medical Mycology
IS - 5
ER -