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Molecular techniques in phytoplankton research: from allozyme electrophoresis to genomics. / De Bruin, A.; Ibelings, B.W.; Van Donk, E.

In: Hydrobiologia, Vol. 491, No. 1-3, 2003, p. 47-63.

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@article{7f4b863bd622487caebd308056b58d9e,
title = "Molecular techniques in phytoplankton research: from allozyme electrophoresis to genomics",
abstract = "Molecular techniques have become a valuable tool in phytoplankton studies over the past decades. The appropriate choice of a technique from an increasing array of methods can be rather complex, because different techniques are suitable for different questions or problems in ecology and evolution. Each technique has its particular strengths and weaknesses and is based upon different (theoretical) assumptions. Our aim is to give a better insight in the (correct) use of various molecular techniques in phytoplankton research, with special emphasis on the fields of strain identification, differentiation of populations and the establishment of phylogenetic relationships. The basic steps in the development of molecular techniques like allozyme electrophoresis, RFLP, DGGE, SSCP, RAPD, AFLP and microsatellites, and the application of these techniques in phytoplankton research, are discussed. Furthermore, recent developments in molecular biology, that have so far only found limited application in phytoplankton studies, such as single-cell PCR, PCR assays combined with molecular probes (Heteroduplex Mobility Assays or DNA arrays), Real-time PCR, complete genome sequencing, multi-gene expression studies using microarrays, and Single Nucleotide Polymorphism (SNPs), are discussed. We emphasise the relevance of fundamental and applied molecular studies on phytoplankton for a wider community of ecologists and evolutionary biologists [KEYWORDS: algae, cyanobacteria, genetic variation, phylogenetics, AFLP, microsatellites, microarrays, genomics, review]",
author = "{De Bruin}, A. and B.W. Ibelings and {Van Donk}, E.",
note = "Reporting year: 2003 Metis note: 3201; CL; AFW ; AqE; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2003/DeBruin_ea_3201.pdf",
year = "2003",
doi = "10.1023/A:1024473620792",
language = "English",
volume = "491",
pages = "47--63",
journal = "Hydrobiologia",
issn = "0018-8158",
publisher = "Springer Netherlands",
number = "1-3",

}

RIS

TY - JOUR

T1 - Molecular techniques in phytoplankton research: from allozyme electrophoresis to genomics

AU - De Bruin, A.

AU - Ibelings, B.W.

AU - Van Donk, E.

N1 - Reporting year: 2003 Metis note: 3201; CL; AFW ; AqE; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2003/DeBruin_ea_3201.pdf

PY - 2003

Y1 - 2003

N2 - Molecular techniques have become a valuable tool in phytoplankton studies over the past decades. The appropriate choice of a technique from an increasing array of methods can be rather complex, because different techniques are suitable for different questions or problems in ecology and evolution. Each technique has its particular strengths and weaknesses and is based upon different (theoretical) assumptions. Our aim is to give a better insight in the (correct) use of various molecular techniques in phytoplankton research, with special emphasis on the fields of strain identification, differentiation of populations and the establishment of phylogenetic relationships. The basic steps in the development of molecular techniques like allozyme electrophoresis, RFLP, DGGE, SSCP, RAPD, AFLP and microsatellites, and the application of these techniques in phytoplankton research, are discussed. Furthermore, recent developments in molecular biology, that have so far only found limited application in phytoplankton studies, such as single-cell PCR, PCR assays combined with molecular probes (Heteroduplex Mobility Assays or DNA arrays), Real-time PCR, complete genome sequencing, multi-gene expression studies using microarrays, and Single Nucleotide Polymorphism (SNPs), are discussed. We emphasise the relevance of fundamental and applied molecular studies on phytoplankton for a wider community of ecologists and evolutionary biologists [KEYWORDS: algae, cyanobacteria, genetic variation, phylogenetics, AFLP, microsatellites, microarrays, genomics, review]

AB - Molecular techniques have become a valuable tool in phytoplankton studies over the past decades. The appropriate choice of a technique from an increasing array of methods can be rather complex, because different techniques are suitable for different questions or problems in ecology and evolution. Each technique has its particular strengths and weaknesses and is based upon different (theoretical) assumptions. Our aim is to give a better insight in the (correct) use of various molecular techniques in phytoplankton research, with special emphasis on the fields of strain identification, differentiation of populations and the establishment of phylogenetic relationships. The basic steps in the development of molecular techniques like allozyme electrophoresis, RFLP, DGGE, SSCP, RAPD, AFLP and microsatellites, and the application of these techniques in phytoplankton research, are discussed. Furthermore, recent developments in molecular biology, that have so far only found limited application in phytoplankton studies, such as single-cell PCR, PCR assays combined with molecular probes (Heteroduplex Mobility Assays or DNA arrays), Real-time PCR, complete genome sequencing, multi-gene expression studies using microarrays, and Single Nucleotide Polymorphism (SNPs), are discussed. We emphasise the relevance of fundamental and applied molecular studies on phytoplankton for a wider community of ecologists and evolutionary biologists [KEYWORDS: algae, cyanobacteria, genetic variation, phylogenetics, AFLP, microsatellites, microarrays, genomics, review]

U2 - 10.1023/A:1024473620792

DO - 10.1023/A:1024473620792

M3 - Article

VL - 491

SP - 47

EP - 63

JO - Hydrobiologia

JF - Hydrobiologia

SN - 0018-8158

IS - 1-3

ER -

ID: 184687