Multilocus microsatellite analysis of European and African Candida glabrata isolates

V. Chillemi; C. Lo Passo; A. D. van Diepeningen; S. Rharmitt; D. Delfino; A. Cascio; N. E. Nnadi; B. D. Cilo; P. Sampaio; H. J. Tietz; J. Peman; G. Criseo; O. Romeo; F. Scordino

doi:10.1007/s10096-016-2610-3

Multilocus microsatellite analysis of European and African Candida glabrata isolates

V. Chillemi, C. Lo Passo, A. D. van Diepeningen, S. Rharmitt, D. Delfino, A. Cascio, N. E. Nnadi, B. D. Cilo, P. Sampaio, H. J. Tietz, J. Peman, G. Criseo, O. Romeo, F. Scordino

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Abstract

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.

Original language	English
Pages (from-to)	885-892
Number of pages	8
Journal	European Journal of Clinical Microbiology & Infectious Diseases
Volume	35
Issue number	6
DOIs	https://doi.org/10.1007/s10096-016-2610-3
Publication status	Published - 2016

Keywords

epidemiology

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Chillemi, V., Lo Passo, C., van Diepeningen, A. D., Rharmitt, S., Delfino, D., Cascio, A., Nnadi, N. E., Cilo, B. D., Sampaio, P., Tietz, H. J., Peman, J., Criseo, G., Romeo, O., & Scordino, F. (2016). Multilocus microsatellite analysis of European and African Candida glabrata isolates. European Journal of Clinical Microbiology & Infectious Diseases, 35(6), 885-892. https://doi.org/10.1007/s10096-016-2610-3

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title = "Multilocus microsatellite analysis of European and African Candida glabrata isolates",

abstract = "This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.",

keywords = "epidemiology",

author = "V. Chillemi and {Lo Passo}, C. and {van Diepeningen}, {A. D.} and S. Rharmitt and D. Delfino and A. Cascio and Nnadi, {N. E.} and Cilo, {B. D.} and P. Sampaio and Tietz, {H. J.} and J. Peman and G. Criseo and O. Romeo and F. Scordino",

note = "ISI Document Delivery No.: DN4GN Times Cited: 0 Cited Reference Count: 20 Chillemi, V. Lo Passo, C. van Diepeningen, A. D. Rharmitt, S. Delfino, D. Cascio, A. Nnadi, N. E. Cilo, B. D. Sampaio, P. Tietz, H. -J. Peman, J. Criseo, G. Romeo, O. Scordino, F. EU Mare Nostrum (EUMN-III Call) program of the European Union [2011-4050/001-EMA2]; EUMN program [F.S. 1.04.11.01 UORI] This research was supported in part by the EU Mare Nostrum (EUMN-III Call) program of the European Union, grant agreement number 2011-4050/001-EMA2. Dr Sanae Rharmitt was the recipient of a scholarship (10 months) signed within the EUMN program for PhD students (F.S. 1.04.11.01 UORI) under the supervision of Prof Orazio Romeo. Springer New york",

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Chillemi, V, Lo Passo, C, van Diepeningen, AD, Rharmitt, S, Delfino, D, Cascio, A, Nnadi, NE, Cilo, BD, Sampaio, P, Tietz, HJ, Peman, J, Criseo, G, Romeo, O & Scordino, F 2016, 'Multilocus microsatellite analysis of European and African Candida glabrata isolates', European Journal of Clinical Microbiology & Infectious Diseases, vol. 35, no. 6, pp. 885-892. https://doi.org/10.1007/s10096-016-2610-3

TY - JOUR

T1 - Multilocus microsatellite analysis of European and African Candida glabrata isolates

AU - Chillemi, V.

AU - Lo Passo, C.

AU - van Diepeningen, A. D.

AU - Rharmitt, S.

AU - Delfino, D.

AU - Cascio, A.

AU - Nnadi, N. E.

AU - Cilo, B. D.

AU - Sampaio, P.

AU - Tietz, H. J.

AU - Peman, J.

AU - Criseo, G.

AU - Romeo, O.

AU - Scordino, F.

N1 - ISI Document Delivery No.: DN4GN Times Cited: 0 Cited Reference Count: 20 Chillemi, V. Lo Passo, C. van Diepeningen, A. D. Rharmitt, S. Delfino, D. Cascio, A. Nnadi, N. E. Cilo, B. D. Sampaio, P. Tietz, H. -J. Peman, J. Criseo, G. Romeo, O. Scordino, F. EU Mare Nostrum (EUMN-III Call) program of the European Union [2011-4050/001-EMA2]; EUMN program [F.S. 1.04.11.01 UORI] This research was supported in part by the EU Mare Nostrum (EUMN-III Call) program of the European Union, grant agreement number 2011-4050/001-EMA2. Dr Sanae Rharmitt was the recipient of a scholarship (10 months) signed within the EUMN program for PhD students (F.S. 1.04.11.01 UORI) under the supervision of Prof Orazio Romeo. Springer New york

PY - 2016

Y1 - 2016

N2 - This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.

AB - This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was MG-9, which was frequently found in both European and African isolates. In conclusion, the data reported here emphasize the role of specific C. glabrata genotypes in human infections for at least some decades and highlight the widespread distribution of some isolates, which seem to be more able to cause disease than others.

KW - epidemiology

U2 - 10.1007/s10096-016-2610-3

DO - 10.1007/s10096-016-2610-3

M3 - Article

SN - 0934-9723

VL - 35

SP - 885

EP - 892

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

IS - 6

ER -

Multilocus microsatellite analysis of European and African Candida glabrata isolates

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