Multilocus sequence typing of Candida albicans isolates from Burn Intensive Care Unit (BICU) in Iran

Mohammad H Afsarian, Hamid Badali, Teun Boekhout, Tahereh Shokohi, Farzad Katiraee

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    31 Citations (Scopus)


    Burn intensive care unit patients are specifically exposed to deep-seated nosocomial infections caused by Candida albicans. Superficial carriage of C. albicans is a potential source of infection and dissemination, and typing methods could be useful to trace the different isolates. Multilocus sequence typing is a powerful genotyping method for pathogenic microorganisms, including Candida albicans. Thirty clinical isolates of C. albicans obtained from 22 patients that admitted to the Burn Intensive Care Unit (BICU) from a burn hospital at Sari, Mazandaran state, Iran were epidemiologically studied by multilocus sequence typing (MLST). Seventy five variable nucleotide sites were found. Sixty two alleles were identified among the seven loci of the C. albicans isolates and one new allele was obtained. Eighteen Diploid Sequence Types (DSTs) were identified and among those 10 were new. These isolates belonged to nine clonal clusters (CCs) while two isolates occurred as singletons. Eleven (36.7%) isolates belonged to CC 124 after eBURST analysis and 13 isolates (43.3%) were assigned to clade 4. Approximately 17% of the 30 isolates belonged to clade 1 (CC 69 and CC766). Isolates from several patients with burns were found to be genetically related. Some patients yielded multiple isolates with identical DSTs suggest colonization or infection caused by cross contamination between patients. Therefore, isolates that show identical or similar allelic profiles are presumed to be identical or closely related and may use to evaluate the genetic relationships between isolates from a specific environment such as BICU.

    Original languageEnglish
    JournalJournal of Medical Microbiology
    Publication statusPublished - 16 Jan 2015


    Dive into the research topics of 'Multilocus sequence typing of Candida albicans isolates from Burn Intensive Care Unit (BICU) in Iran'. Together they form a unique fingerprint.

    Cite this