TY - JOUR
T1 - New endemic Fusarium species hitch-hiking with pathogenic Fusarium strains causing Panama disease in small-holder banana plots in Indonesia
AU - Maryani, N.
AU - Sandoval-Denis, M.
AU - Lombard, L.
AU - Crous, P. W.
AU - Kema, G. H.J.
N1 - Funding Information:
Acknowledgements This research was supported by the KNAW-SPIN Project, ‘The Indonesian banana: Protecting a staple food from Panama disease collapse and exploiting its genetic diversity for discovery research’. NM was also supported by a DIKTI (Directorate General of Higher Education) Scholarship, Ministry of Research, Technology and Higher Education, Indonesia. Banana research at Wageningen University and Research is financially supported by the Dutch Dioraphte Foundation. Rahan Meristem, Israel, is gratefully acknowledged for supporting our trials by providing unlimited numbers of Cavendish banana plants.
Publisher Copyright:
© 2019 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019/12
Y1 - 2019/12
N2 - Fusarium species are well known for their abundance, diversity and cosmopolitan life style. Many members of the genus Fusarium are associated with plant hosts, either as plant pathogens, secondary invaders, saprotrophs, and/or endophytes. We previously studied the diversity of Fusarium species in the Fusarium oxysporum species complex (FOSC) associated with Fusarium wilt of banana in Indonesia. In that study, several Fusarium species not belonging to the FOSC were found to be associated with Fusarium wilt of banana. These Fusarium isolates belonged to three Fusarium species complexes, which included the Fusarium fujikuroi species complex (FFSC), Fusarium incarnatum-equiseti species complex (FIESC) and the Fusarium sambucinum species complex (FSSC). Using a multi-gene phylogeny that included partial fragments of the beta-tubulin (tub), calmodulin (cmdA), translation elongation factor 1-alpha (tef1), the internal transcribed spacer region of the rDNA (ITS), the large subunit of the rDNA (LSU), plus the RNA polymerase II large subunit (rpb1) and second largest subunit (rpb2) genes, we were able to identify and characterise several of these as new Fusarium species in the respective species complexes identified in this study.
AB - Fusarium species are well known for their abundance, diversity and cosmopolitan life style. Many members of the genus Fusarium are associated with plant hosts, either as plant pathogens, secondary invaders, saprotrophs, and/or endophytes. We previously studied the diversity of Fusarium species in the Fusarium oxysporum species complex (FOSC) associated with Fusarium wilt of banana in Indonesia. In that study, several Fusarium species not belonging to the FOSC were found to be associated with Fusarium wilt of banana. These Fusarium isolates belonged to three Fusarium species complexes, which included the Fusarium fujikuroi species complex (FFSC), Fusarium incarnatum-equiseti species complex (FIESC) and the Fusarium sambucinum species complex (FSSC). Using a multi-gene phylogeny that included partial fragments of the beta-tubulin (tub), calmodulin (cmdA), translation elongation factor 1-alpha (tef1), the internal transcribed spacer region of the rDNA (ITS), the large subunit of the rDNA (LSU), plus the RNA polymerase II large subunit (rpb1) and second largest subunit (rpb2) genes, we were able to identify and characterise several of these as new Fusarium species in the respective species complexes identified in this study.
KW - Indonesia
KW - new species
KW - non-pathogenic
KW - phylogeny
KW - species complex
UR - http://www.scopus.com/inward/record.url?scp=85069602975&partnerID=8YFLogxK
U2 - 10.3767/persoonia.2019.43.02
DO - 10.3767/persoonia.2019.43.02
M3 - Article
AN - SCOPUS:85069602975
SN - 0031-5850
VL - 43
SP - 48
EP - 69
JO - Persoonia: Molecular Phylogeny and Evolution of Fungi
JF - Persoonia: Molecular Phylogeny and Evolution of Fungi
ER -