TY - JOUR
T1 - New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov.
AU - Bezerra, Jadson D.P.
AU - Sandoval-Denis, Marcelo
AU - Paiva, Laura M.
AU - Silva, Gladstone A.
AU - Groenewald, Johannes Z.
AU - Souza-Motta, Cristina M.
AU - Crous, Pedro W.
N1 - Funding Information:
We thank the Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) (Process 203132/2014-9), the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) and the Funda??o de Amparo ? Ci?ncia e Tecnologia de Pernambuco (FACEPE) of Brazil for financial support and scholarships. We also thank Konstanze Bensch and David L. Hawksworth for the valuable comments and suggestions to improve the manuscript. We extend our thanks to the Universidade Federal de Pernambuco and to the technical staff, Eliane Silva-Nogueira and Luan Amim from the URM Culture Collection, and to Marjan Vermaas, Arien van Iperen and Mieke Starink-Willemse from the Westerdijk Fungal Biodiversity Institute. We also thank Tamara Caldas, Greicilene Albuquerque, Gianne Rizzuto, Karla Freire and other students of the Laborat?rio de Micologia Ambiental/UFPE for their technical help and processing of samples.
Publisher Copyright:
© 2017 International Mycological Association.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Brazil harbours a unique ecosystem, the Caatinga, which belongs to the tropical dry forest biome. This region has an important diversity of organisms, and recently several new fungal species have been described from different hosts and substrates within it. During a survey of fungal endophyte diversity from cacti in this forest, we isolated cladosporium-like fungi that were subjected to morphological and multigene phylogenetic analyses including actA, ITS, LSU, rpb2 and tub2 gene sequences. Based on these analyses we identified two new species belonging to the genus Toxicocladosporium, described here as T. cacti and T. immaculatum spp. nov., isolated from Pilosocereus gounellei subsp. gounellei and Melocactus zehntneri, respectively. To improve the species recognition and assess species diversity in Toxicocladosporium we studied all ex-type strains of the genus, for which actA, rpb2 and tub2 barcodes were also generated. After phylogenetic reconstruction using five loci, we differentiated 13 species in the genus. Toxicocladosporium velox and T. chlamydosporum are synonymized based on their phylogenetic position and limited number of unique nucleotide differences. Six strains previously assigned to T. leucadendri, including the ex-type strain (CBS 131317) of that species, were found to belong to an undescribed genus here named as Neocladosporium gen. nov., with N. leucadendri comb. nov. as type species. Furthermore, this study proposes the actA, ITS, rpb2 and tub2 as main phylogenetic loci to recognise Toxicocladosporium species.
AB - Brazil harbours a unique ecosystem, the Caatinga, which belongs to the tropical dry forest biome. This region has an important diversity of organisms, and recently several new fungal species have been described from different hosts and substrates within it. During a survey of fungal endophyte diversity from cacti in this forest, we isolated cladosporium-like fungi that were subjected to morphological and multigene phylogenetic analyses including actA, ITS, LSU, rpb2 and tub2 gene sequences. Based on these analyses we identified two new species belonging to the genus Toxicocladosporium, described here as T. cacti and T. immaculatum spp. nov., isolated from Pilosocereus gounellei subsp. gounellei and Melocactus zehntneri, respectively. To improve the species recognition and assess species diversity in Toxicocladosporium we studied all ex-type strains of the genus, for which actA, rpb2 and tub2 barcodes were also generated. After phylogenetic reconstruction using five loci, we differentiated 13 species in the genus. Toxicocladosporium velox and T. chlamydosporum are synonymized based on their phylogenetic position and limited number of unique nucleotide differences. Six strains previously assigned to T. leucadendri, including the ex-type strain (CBS 131317) of that species, were found to belong to an undescribed genus here named as Neocladosporium gen. nov., with N. leucadendri comb. nov. as type species. Furthermore, this study proposes the actA, ITS, rpb2 and tub2 as main phylogenetic loci to recognise Toxicocladosporium species.
KW - Cladosporiaceae
KW - Endophytic fungi
KW - Multigene phylogeny
KW - Taxonomy
UR - http://www.scopus.com/inward/record.url?scp=85023606504&partnerID=8YFLogxK
U2 - 10.5598/imafungus.2017.08.01.06
DO - 10.5598/imafungus.2017.08.01.06
M3 - Article
AN - SCOPUS:85023606504
SN - 2210-6340
VL - 8
SP - 77
EP - 97
JO - IMA Fungus
JF - IMA Fungus
IS - 1
ER -