Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture

Sjoerd Jd Tjalsma; Wouter de Laat

doi:10.1016/j.gde.2020.10.002

Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture

Sjoerd Jd Tjalsma, Wouter de Laat

Hubrecht Institute

Research output: Contribution to journal/periodical › Book/Film/Article review › Scientific

6 Citations (Scopus)

Abstract

Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.

Original language	English
Pages (from-to)	10-17
Number of pages	8
Journal	Current Opinion in Genetics & Development
Volume	67
DOIs	https://doi.org/10.1016/j.gde.2020.10.002
Publication status	E-pub ahead of print - 18 Nov 2020

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10.1016/j.gde.2020.10.002

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abstract = "Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.",

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AU - de Laat, Wouter

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AB - Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.

U2 - 10.1016/j.gde.2020.10.002

DO - 10.1016/j.gde.2020.10.002

M3 - Book/Film/Article review

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VL - 67

SP - 10

EP - 17

JO - Current Opinion in Genetics & Development

JF - Current Opinion in Genetics & Development

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Novel orthogonal methods to uncover the complexity and diversity of nuclear architecture

Abstract

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