Opportunities and limitations of reduced representation bisulfite sequencing in plant ecological epigenomics

Ovidiu Paun (Corresponding author), Koen J. F. Verhoeven, Christina L. Richards

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Abstract

Investigating the features and implications of epigenetic mechanisms across the breadth of organisms and ecosystems is important for understanding the ecological relevance of epigenetics. Several cost-effective reduced representation bisulfite sequencing approaches (RRBS) have been recently developed and applied to different organisms that lack a well annotated reference genome. These new approaches improve the assessment of epigenetic diversity in ecological settings and may provide functional insights. We assess here the opportunities and limitations of RRBS in nonmodel plant species. Well thought out experimental designs that include complementary gene expression studies, and the improvement of genomics resources for the target group, promise to maximize the effect of future RRBS studies.
Original languageEnglish
Pages (from-to)738-742
JournalNew Phytologist
Volume221
Issue number2
DOIs
Publication statusPublished - Jan 2019

Keywords

  • international
  • bsRADseq
  • DNA methylation
  • ecological genomics
  • epiGBS
  • reduced representation bisulfite sequencing (RRBS)
  • response to environment
  • adaptation
  • bisulfite sequencing

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