The members of subdivision 1 Acidobacterium were grown at different pH values in a new medium formulation named PSYL 5 includes sucrose, as a carbon source and other compounds (such as KH2PO4 and MgSO4.7H2O). Growth rate was nearly constant at pH 5 and declined at pH 3–4 and 6–7. However, it was found that the effects involving good C/N ratios and pH on the growth of members of subdivision 1 Acidobacterium were significant, and the strongest effect was given by this conditions at pH 5. In additional, incubation results of 48, 72, 96 and 120 h were relatively shorter as compared to other media earlier described for members of subdivision 1 of the phylum Acidobacteria on solid laboratory media.
Campanharo, J. C., Kielak, A. M., Castellane, T. C., Kuramae, E. E., & Lemos, E. G. (2016). Optimized medium culture for Acidobacteia subdivision 1 strains. FEMS Microbiology Letters, 363(21), [fnw245]. https://doi.org/10.1093/femsle/fnw245