Oral mucosal organoids as a potential platform for personalized cancer therapy

Else Driehuis, Sigrid Kolders, Sacha Spelier, Kadi Lohmussaar, Stefan M Willems, Lot A Devriese, Remco de Bree, Emma J de Ruiter, Jeroen Korving, Harry Begthel, Johan H Van Es, Veerle Geurts, Gui-Wei He, Richard H van Jaarsveld, Rurika Oka, Mauro J Muraro, Judith Vivie, Maurice M J M Zandvliet, Antoni P A Hendrickx, Nino IakobachviliPriya Sridevi, Onno Kranenburg, Ruben van Boxtel, Geert J P L Kops, David A Tuveson, Peter J Peters, Alexander van Oudenaarden, Hans Clevers

Research output: Contribution to journal/periodicalArticleScientificpeer-review

218 Citations (Scopus)

Abstract

Previous studies have described that tumor organoids can capture the diversity of defined human carcinoma types. Here, we describe conditions for long-term culture of human mucosal organoids. Using this protocol, a panel of 31 head and neck squamous cell carcinoma (HNSCC)-derived organoid lines was established. This panel recapitulates genetic and molecular characteristics previously described for HNSCC. Organoids retain their tumorigenic potential upon xenotransplantation. We observe differential responses to a panel of drugs including cisplatin, carboplatin, cetuximab and radiotherapy in vitro. Drug screens reveals selective sensitivity to targeted drugs that are not normally used in the treatment of HNSCC patients. These observations may inspire a personalized approach to the management of HNSCC and expand the repertoire of HNSCC drugs.

Original languageEnglish
JournalCancer Discovery
Early online date03 May 2019
DOIs
Publication statusPublished - Jul 2019

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