Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology

TY - JOUR

T1 - Out with the old, in with the new

T2 - reassessing morpholino knockdowns in light of genome editing technology

AU - Schulte-Merker, Stefan

AU - Stainier, Didier Y R

N1 - © 2014. Published by The Company of Biologists Ltd.

PY - 2014/8

Y1 - 2014/8

N2 - Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.

AB - Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.

KW - Animals

KW - Genetic Techniques

KW - Genome

KW - Morpholinos

KW - Mutation

KW - Phenotype

KW - Zebrafish

U2 - 10.1242/dev.112003

DO - 10.1242/dev.112003

M3 - Article

C2 - 25100652

SN - 0950-1991

VL - 141

SP - 3103

EP - 3104

JO - Development

JF - Development

IS - 16

ER -