Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

Mireille M J P E Sthijns; Marlon J Jetten; Sami G Mohammed; Sandra M H Claessen; Rick H W de Vries; Adam Stell; Denise F A de Bont; Marten A Engelse; Didem Mumcuoglu; Clemens A van Blitterswijk; Patricia Y W Dankers; Eelco J P de Koning; Aart A van Apeldoorn; Vanessa L S LaPointe

doi:10.1016/j.biomaterials.2020.120449

Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

Mireille M J P E Sthijns, Marlon J Jetten, Sami G Mohammed, Sandra M H Claessen, Rick H W de Vries, Adam Stell, Denise F A de Bont, Marten A Engelse, Didem Mumcuoglu, Clemens A van Blitterswijk, Patricia Y W Dankers, Eelco J P de Koning, Aart A van Apeldoorn, Vanessa L S LaPointe

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.

Original language	English
Pages (from-to)	120449
Journal	Biomaterials
Volume	267
DOIs	https://doi.org/10.1016/j.biomaterials.2020.120449
Publication status	Published - Jan 2021

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Sthijns, M. M. J. P. E., Jetten, M. J., Mohammed, S. G., Claessen, S. M. H., de Vries, R. H. W., Stell, A., de Bont, D. F. A., Engelse, M. A., Mumcuoglu, D., van Blitterswijk, C. A., Dankers, P. Y. W., de Koning, E. J. P., van Apeldoorn, A. A., & LaPointe, V. L. S. (2021). Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials. Biomaterials, 267, 120449. https://doi.org/10.1016/j.biomaterials.2020.120449

@article{3b62cecb81fa4517b6273f28429e3013,

title = "Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials",

abstract = "The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.",

author = "Sthijns, {Mireille M J P E} and Jetten, {Marlon J} and Mohammed, {Sami G} and Claessen, {Sandra M H} and {de Vries}, {Rick H W} and Adam Stell and {de Bont}, {Denise F A} and Engelse, {Marten A} and Didem Mumcuoglu and {van Blitterswijk}, {Clemens A} and Dankers, {Patricia Y W} and {de Koning}, {Eelco J P} and {van Apeldoorn}, {Aart A} and LaPointe, {Vanessa L S}",

year = "2021",

month = jan,

doi = "10.1016/j.biomaterials.2020.120449",

language = "English",

volume = "267",

pages = "120449",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier B.V.",

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Sthijns, MMJPE, Jetten, MJ, Mohammed, SG, Claessen, SMH, de Vries, RHW, Stell, A, de Bont, DFA, Engelse, MA, Mumcuoglu, D, van Blitterswijk, CA, Dankers, PYW, de Koning, EJP, van Apeldoorn, AA & LaPointe, VLS 2021, 'Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials', Biomaterials, vol. 267, pp. 120449. https://doi.org/10.1016/j.biomaterials.2020.120449

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T1 - Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

AU - Sthijns, Mireille M J P E

AU - Jetten, Marlon J

AU - Mohammed, Sami G

AU - Claessen, Sandra M H

AU - de Vries, Rick H W

AU - Stell, Adam

AU - de Bont, Denise F A

AU - Engelse, Marten A

AU - Mumcuoglu, Didem

AU - van Blitterswijk, Clemens A

AU - Dankers, Patricia Y W

AU - de Koning, Eelco J P

AU - van Apeldoorn, Aart A

AU - LaPointe, Vanessa L S

PY - 2021/1

Y1 - 2021/1

N2 - The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.

AB - The clinical success rate of islet transplantation, namely independence from insulin injections, is limited by factors that lead to graft failure, including inflammation, acute ischemia, acute phase response, and insufficient vascularization. The ischemia and insufficient vascularization both lead to high levels of oxidative stress, which are further aggravated by islet encapsulation, inflammation, and undesirable cell-biomaterial interactions. To identify biomaterials that would not further increase damaging oxidative stress levels and that are also suitable for manufacturing a beta cell encapsulation device, we studied five clinically approved polymers for their effect on oxidative stress and islet (alpha and beta cell) function. We found that 300 poly(ethylene oxide terephthalate) 55/poly(butylene terephthalate) 45 (PEOT/PBT300) was more resistant to breakage and more elastic than other biomaterials, which is important for its immunoprotective function. In addition, it did not induce oxidative stress or reduce viability in the MIN6 beta cell line, and even promoted protective endogenous antioxidant expression over 7 days. Importantly, PEOT/PBT300 is one of the biomaterials we studied that did not interfere with insulin secretion in human islets.

U2 - 10.1016/j.biomaterials.2020.120449

DO - 10.1016/j.biomaterials.2020.120449

M3 - Article

C2 - 33129188

SN - 0142-9612

VL - 267

SP - 120449

JO - Biomaterials

JF - Biomaterials

ER -

Oxidative stress in pancreatic alpha and beta cells as a selection criterion for biocompatible biomaterials

Abstract

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