Personalized Proteome Profiles of Healthy and Tumor Human Colon Organoids Reveal Both Individual Diversity and Basic Features of Colorectal Cancer

Alba Cristobal, Henk W P van den Toorn, Marc van de Wetering, Hans Clevers, Albert J R Heck, Shabaz Mohammed

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

Diseases at the molecular level are complex and patient dependent, necessitating development of strategies that enable precision treatment to optimize clinical outcomes. Organoid technology has recently been shown to have the potential to recapitulate the in vivo characteristics of the original individual's tissue in a three-dimensional in vitro culture system. Here, we present a quantitative mass-spectrometry-based proteomic analysis and a comparative transcriptomic analysis of human colorectal tumor and healthy organoids derived, in parallel, from seven patients. Although gene and protein signatures can be derived to distinguish the tumor organoid population from healthy organoids, our data clearly reveal that each patient possesses a distinct organoid signature at the proteomic level. We demonstrate that a personalized patient-specific organoid proteome profile can be related to the diagnosis of a patient and with future development contribute to the generation of personalized therapies.

Original languageEnglish
Pages (from-to)263-274
Number of pages12
JournalCell Reports
Volume18
Issue number1
DOIs
Publication statusPublished - 03 Jan 2017

Keywords

  • Colon
  • Colorectal Neoplasms
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Genetic Variation
  • Humans
  • Organoids
  • Proteome
  • Proteomics
  • Transcriptome
  • Wnt Signaling Pathway
  • Journal Article
  • Research Support, Non-U.S. Gov't

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