Phenotypic and molecular diversity of Meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation

Laura Corte, Raffaella di Cagno, Marizeth Groenewald, Luca Roscini, Claudia Colabella, Marco Gobbetti, Gianluigi Cardinali

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    37 Citations (Scopus)

    Abstract

    Meyerozyma guilliermondii is a yeast species widely isolated from several natural environments and from fruit; in medical microbiology it is known as the teleomorph of the opportunistic pathogen Candida guilliermondii, which causes about 2% of the human blood infections. This yeast is also promising in a variety of biotechnological applications as vitamins production and post-harvest control. The question if isolates from different sources are physiologically and genetically similar, or if the various environments induced significant differences, is crucial for the understanding of this species structure and to select strains appropriate for each application. This question was addressed using LSU and ITS sequencing for taxonomic assignment, i-SSR (GACA4) for the molecular characterization and FTIR for the metabolomic fingerprint. All data showed that fruit and environmental isolates cluster separately with a general good agreement between metabolomics and molecular analysis. An additional RAPD analysis was able to discriminate strains according to the isolation position within the pineapple fruit. Although all strains are members of the M. guilliermondii species according to the current standards, the distribution of large variability detected suggests that some specialization occurred in the niches inhabited by this yeast and that food related strains can be differentiated from the medical isolates.
    Original languageEnglish
    Pages (from-to)206-215
    JournalFood Microbiology
    Volume48
    DOIs
    Publication statusPublished - Jun 2015

    Keywords

    • Food environment
    • Fruits
    • Meyerozyma guilliermondii
    • Candida guilliermondii
    • Species diversity
    • Yeast

    Fingerprint

    Dive into the research topics of 'Phenotypic and molecular diversity of Meyerozyma guilliermondii strains isolated from food and other environmental niches, hints for an incipient speciation'. Together they form a unique fingerprint.

    Cite this