Phylogenetic analysis and substrate specificity of GH2 beta-mannosidases from Aspergillus species

S.K. Reddy, A. Rosengren, S. Klaubauf, T. Kulkarni, E.N. Karlsson, R.P. de Vries, H. Stalbrand

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    16 Citations (Scopus)

    Abstract

    Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised beta-mannosidases from other organisms, clusters putative Aspergillus beta-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched beta-mannosides like mannobiose. A 3D-model of MndB provides further insight.
    Original languageEnglish
    Pages (from-to)3444-3449
    JournalFEBS Letters
    Volume587
    Issue number21
    DOIs
    Publication statusPublished - 2013

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