Phylogenetic analysis and substrate specificity of GH2 beta-mannosidases from Aspergillus species

TY - JOUR

T1 - Phylogenetic analysis and substrate specificity of GH2 beta-mannosidases from Aspergillus species

AU - Reddy, S.K.

AU - Rosengren, A.

AU - Klaubauf, S.

AU - Kulkarni, T.

AU - Karlsson, E.N.

AU - de Vries, R.P.

AU - Stalbrand, H.

N1 - Reporting year: 2013

PY - 2013

Y1 - 2013

N2 - Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised beta-mannosidases from other organisms, clusters putative Aspergillus beta-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched beta-mannosides like mannobiose. A 3D-model of MndB provides further insight.

AB - Phylogenetic analysis of glycoside hydrolase family 2 including Aspergillus sequences and characterised beta-mannosidases from other organisms, clusters putative Aspergillus beta-mannosidases in two distinct clades (A and B). Aspergillus species have at least one paralog in each of the two clades. It appears that clade A members are extracellular and clade B members intracellular. Substrate specificity analysis of MndA of Aspergillus niger (clade A) and MndB of Aspergillus nidulans (clade B) show that MndB, in contrast to MndA, does not hydrolyse polymeric mannan and has probably evolved to hydrolyse small unbranched beta-mannosides like mannobiose. A 3D-model of MndB provides further insight.

U2 - 10.1016/j.febslet.2013.08.029

DO - 10.1016/j.febslet.2013.08.029

M3 - Article

SN - 0014-5793

VL - 587

SP - 3444

EP - 3449

JO - FEBS Letters

JF - FEBS Letters

IS - 21

ER -