Phylogenetic characterization of phosphatase-expressing bacterial communities in Baltic Sea sediments

Anne Steenbergh, Paul Bodelier, H.L. Hoogveld, C.P Slomp, H.J. Laanbroek

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Abstract

Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria.
Original languageEnglish
Article numberME14074
Pages (from-to)192-195
JournalMicrobes and Environments
Volume30
Issue number2
Early online date28 Mar 2015
DOIs
Publication statusPublished - 2015

Keywords

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