Abstract
Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria.
Original language | English |
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Article number | ME14074 |
Pages (from-to) | 192-195 |
Journal | Microbes and Environments |
Volume | 30 |
Issue number | 2 |
Early online date | 28 Mar 2015 |
DOIs | |
Publication status | Published - 2015 |
Keywords
- national
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Data from: Phylogenetic characterization of phosphatase-expressing bacterial communities in Baltic Sea sediments[fasta]
Steenbergh, A. K. (Creator), Marine Data Archive, 10 Dec 2014
http://mda.vliz.be/mda/directlink.php?fid=VLIZ_00000337_1418222111
Dataset
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Data from: Phylogenetic characterization of phosphatase-expressing bacterial communities in Baltic Sea sediments[images of DGGE gels with numbered bands]
Steenbergh, A. K. (Creator), Marine Data Archive, 10 Dec 2014
http://mda.vliz.be/mda/directlink.php?fid=VLIZ_00000337_1418222539
Dataset