Phosphate release from sediments hampers the remediation of aquatic systems from a eutrophic state. Microbial phosphatases in sediments release phosphorus during organic matter degradation. Despite the important role of phosphatase-expressing bacteria, the identity of these bacteria in sediments is largely unknown. We herein presented a culture-independent method to phylogenetically characterize phosphatase-expressing bacteria in sediments. We labeled whole-cell extracts of Baltic Sea sediments with an artificial phosphatase substrate and sorted phosphatase-expressing cells with a flow cytometer. Their phylogenetic affiliation was determined by Denaturing Gradient Gel Electrophoresis. The phosphatase-expressing bacterial community coarsely reflected the whole-cell bacterial community, with a similar dominance of Alphaproteobacteria.
Data from: Phylogenetic characterization of phosphatase-expressing bacterial communities in Baltic Sea sediments[images of DGGE gels with numbered bands]
Steenbergh, A. K. (Creator), Marine Data Archive, 10 Dec 2014
Steenbergh, A., Bodelier, P., Hoogveld, H. L., Slomp, C. P., & Laanbroek, H. J. (2015). Phylogenetic characterization of phosphatase-expressing bacterial communities in Baltic Sea sediments. Microbes and Environments, 30(2), 192-195. [ME14074]. https://doi.org/10.1264/jsme2.ME14074