Phylogenetic composition and properties of bacteria coexisting with the fungus Hypholoma fasciculare in decaying wood

TY - JOUR

T1 - Phylogenetic composition and properties of bacteria coexisting with the fungus Hypholoma fasciculare in decaying wood

AU - Valášková, V.

AU - De Boer, W.

AU - Klein Gunnewiek, P.J.A.

AU - Pospíšek, M.

AU - Baldrian, P.

N1 - Reporting year: 2009 Metis note: 4638;CTE; ME; file:///L:/Endnotedatabases/NIOOPUB/pdfs/PDFS2009\Valaskova_ea_4638.pdf

PY - 2009

Y1 - 2009

N2 - White-rot fungi are major degraders of woody materials in terrestrial environments because of their ability to decompose lignin. However, little is known on the possible associations of white-rot fungi with other microorganisms during wood decay. We investigated the numbers, community composition and functional traits of bacteria present in natural wood samples under advanced decay by the white-rot basidiomycete Hypholoma fasciculare. The wood samples contained high numbers of cultivable bacteria (0.2–8 109 colony forming units (CFU) per g of dry wood). Most cultivable bacteria belonged to Proteobacteria and Acidobacteria (75% and 23% of sequences, respectively). The same phyla were also found to be dominant (59% and 23%, respectively) using a non-culturable quantification technique, namely, direct cloning and sequencing of 16sRNA genes extracted from wood. Bacteria that could be subcultured consisted of acid-tolerant strains that seemed to rely on substrates released by lignocellulolytic enzyme activities of the fungus. There were no indications for antagonism (antibiosis) of the bacteria against the fungus.

AB - White-rot fungi are major degraders of woody materials in terrestrial environments because of their ability to decompose lignin. However, little is known on the possible associations of white-rot fungi with other microorganisms during wood decay. We investigated the numbers, community composition and functional traits of bacteria present in natural wood samples under advanced decay by the white-rot basidiomycete Hypholoma fasciculare. The wood samples contained high numbers of cultivable bacteria (0.2–8 109 colony forming units (CFU) per g of dry wood). Most cultivable bacteria belonged to Proteobacteria and Acidobacteria (75% and 23% of sequences, respectively). The same phyla were also found to be dominant (59% and 23%, respectively) using a non-culturable quantification technique, namely, direct cloning and sequencing of 16sRNA genes extracted from wood. Bacteria that could be subcultured consisted of acid-tolerant strains that seemed to rely on substrates released by lignocellulolytic enzyme activities of the fungus. There were no indications for antagonism (antibiosis) of the bacteria against the fungus.

U2 - 10.1038/ismej.2009.64

DO - 10.1038/ismej.2009.64

M3 - Article

VL - 3

SP - 1218

EP - 1221

JO - ISME Journal

JF - ISME Journal

SN - 1751-7362

IS - 10

ER -