Phylogenetic diversity of Acidobacteria in a former agricultural soil

TY - JOUR

T1 - Phylogenetic diversity of Acidobacteria in a former agricultural soil

AU - Kielak, A.M.

AU - Pijl, A.S.

AU - Van Veen, J.A.

AU - Kowalchuk, G.A.

N1 - Reporting year: 2009 Metis note: 4417;CTE; TME ; ME; file:///L:/Endnotedatabases/NIOOPUB/pdfs/PDFS2009\Kielak_ea_4417.pdf

PY - 2009

Y1 - 2009

N2 - Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P

AB - Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P

U2 - 10.1038/ismej.2008.113

DO - 10.1038/ismej.2008.113

M3 - Article

SN - 1751-7362

VL - 3

SP - 378

EP - 382

JO - ISME Journal

JF - ISME Journal

IS - 3

ER -