Phylogenetic diversity of Acidobacteria in a former agricultural soil

A.M. Kielak, A.S. Pijl, J.A. Van Veen, G.A. Kowalchuk

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    109 Citations (Scopus)


    Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P
    Original languageEnglish
    Pages (from-to)378-382
    JournalISME Journal
    Issue number3
    Publication statusPublished - 2009


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