Phylogenetic diversity of Acidobacteria in a former agricultural soil

A.M. Kielak; A.S. Pijl; J.A. Van Veen; G.A. Kowalchuk

doi:10.1038/ismej.2008.113

Phylogenetic diversity of Acidobacteria in a former agricultural soil

A.M. Kielak, A.S. Pijl, J.A. Van Veen, G.A. Kowalchuk

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P

Original language	English
Pages (from-to)	378-382
Journal	ISME Journal
Volume	3
Issue number	3
DOIs	https://doi.org/10.1038/ismej.2008.113
Publication status	Published - 2009

Access to Document

10.1038/ismej.2008.113

Embargoed Document

PDF
Final published version, 114 KB
Request copy

Fingerprint Dive into the research topics of 'Phylogenetic diversity of Acidobacteria in a former agricultural soil'. Together they form a unique fingerprint.

Cite this

Kielak, A. M., Pijl, A. S., Van Veen, J. A., & Kowalchuk, G. A. (2009). Phylogenetic diversity of Acidobacteria in a former agricultural soil. ISME Journal, 3(3), 378-382. https://doi.org/10.1038/ismej.2008.113

@article{6dcdc8389ae740e19c9f204e75a6e9b9,

title = "Phylogenetic diversity of Acidobacteria in a former agricultural soil",

abstract = "Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P",

author = "A.M. Kielak and A.S. Pijl and {Van Veen}, J.A. and G.A. Kowalchuk",

note = "Reporting year: 2009 Metis note: 4417;CTE; TME ; ME; file:///L:/Endnotedatabases/NIOOPUB/pdfs/PDFS2009\Kielak_ea_4417.pdf",

year = "2009",

doi = "10.1038/ismej.2008.113",

language = "English",

volume = "3",

pages = "378--382",

journal = "ISME Journal",

issn = "1751-7362",

number = "3",

}

TY - JOUR

T1 - Phylogenetic diversity of Acidobacteria in a former agricultural soil

AU - Kielak, A.M.

AU - Pijl, A.S.

AU - Van Veen, J.A.

AU - Kowalchuk, G.A.

N1 - Reporting year: 2009 Metis note: 4417;CTE; TME ; ME; file:///L:/Endnotedatabases/NIOOPUB/pdfs/PDFS2009\Kielak_ea_4417.pdf

PY - 2009

Y1 - 2009

N2 - Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P

AB - Although Acidobacteria represent the most abundant bacterial phylum in many soils, knowledge of acidobacterial diversity is still rather incomplete. We, therefore, examined the diversity of 16S rRNA genes affiliated with this phylum in a former arable soil via three independent approaches: (1) screening of a fosmid metagenome library for inserts containing Acidobacteria-like 16S rRNA genes; (2) PCR-cloning using general bacterial primers; and (3) PCR-cloning with acidobacterial-specific primers. Bacterial-specific libraries compared rhizosphere versus bulk soil samples, revealing a higher proportion of acidobacterial sequences in bulk soil libraries (P

U2 - 10.1038/ismej.2008.113

DO - 10.1038/ismej.2008.113

M3 - Article

VL - 3

SP - 378

EP - 382

JO - ISME Journal

JF - ISME Journal

SN - 1751-7362

IS - 3

ER -