TY - JOUR
T1 - Physiological and molecular characterization of atypical lipid-dependent Malassezia yeasts from a dog with skin lesions: adaptation to a new host?
AU - Cafarchia, C.
AU - Latrofa, M.S.
AU - Figueredo, L.A.
AU - da Silva Machado, M.L.
AU - Ferreiro, L.
AU - Guillot, J.
AU - Boekhout, T.
AU - Otranto, D.
N1 - Reporting year: 2011
PY - 2011
Y1 - 2011
N2 - Three lipid-dependent Malassezia isolates (here named 114A, 114B and 114C) recovered from a dog with skin lesions were phenotypically and genotypically characterized. All presented ovoid cells and buds formed on a narrow base. Most of the results from physiological tests were consistent with those of Malassezia furfur. The phylogenetic analysis of ITS-1 and LSU nucleotide sequences was concordant in placing all three clinical Malassezia isolates close to M. furfur. However, the phylogenetic data on the chs-2 sequence revealed that clinical isolate 114A is distinct from M. furfur and was closely affiliated to the sequence of M. pachydermatis with high nodal support. In particular, lipid-dependent isolates 114A displayed chs-2 sequences similar (100%) to that of the non-lipid dependent species Malassezia pachydermatis. The presence of the genetic and physiological polymorphisms detected in these three isolates of M. furfur could have resulted from a process of adaptation of this anthropophilic species to a new host. [KEYWORDS: Adaptation, Biological ,Amino Acid Sequence ,Animals ,Cell Nucleus ,Chitin Synthase/*genetics ,DNA, Fungal/genetics ,DNA, Ribosomal Spacer/genetics ,Dermatomycoses/microbiology/*veterinary ,Dog Diseases/*microbiology ,Dogs ,Female ,Genes, Fungal ,Glycerol/analogs & derivatives ,Malassezia/classification/genetics/*isolation & purification ,Molecular Sequence Data ,Phylogeny]
AB - Three lipid-dependent Malassezia isolates (here named 114A, 114B and 114C) recovered from a dog with skin lesions were phenotypically and genotypically characterized. All presented ovoid cells and buds formed on a narrow base. Most of the results from physiological tests were consistent with those of Malassezia furfur. The phylogenetic analysis of ITS-1 and LSU nucleotide sequences was concordant in placing all three clinical Malassezia isolates close to M. furfur. However, the phylogenetic data on the chs-2 sequence revealed that clinical isolate 114A is distinct from M. furfur and was closely affiliated to the sequence of M. pachydermatis with high nodal support. In particular, lipid-dependent isolates 114A displayed chs-2 sequences similar (100%) to that of the non-lipid dependent species Malassezia pachydermatis. The presence of the genetic and physiological polymorphisms detected in these three isolates of M. furfur could have resulted from a process of adaptation of this anthropophilic species to a new host. [KEYWORDS: Adaptation, Biological ,Amino Acid Sequence ,Animals ,Cell Nucleus ,Chitin Synthase/*genetics ,DNA, Fungal/genetics ,DNA, Ribosomal Spacer/genetics ,Dermatomycoses/microbiology/*veterinary ,Dog Diseases/*microbiology ,Dogs ,Female ,Genes, Fungal ,Glycerol/analogs & derivatives ,Malassezia/classification/genetics/*isolation & purification ,Molecular Sequence Data ,Phylogeny]
U2 - 10.3109/13693786.2010.531487
DO - 10.3109/13693786.2010.531487
M3 - Article
SN - 1369-3786
VL - 49
SP - 365
EP - 374
JO - Medical Mycology
JF - Medical Mycology
IS - 4
ER -