Pigeon pea-mediated soil microbial shifts improve agroecosystem multifunctionality in long-term maize–palisade grass intercropping

TY - JOUR

T1 - Pigeon pea-mediated soil microbial shifts improve agroecosystem multifunctionality in long-term maize–palisade grass intercropping

AU - Khoiri, Ahmad Nuruddin

AU - Costa, Nídia Raquel

AU - Crusciol, Carlos Alexandre Costa

AU - Pariz, Cristiano Magalhães

AU - Costa, Ciniro

AU - Calonego, Juliano Carlos

AU - de Castilhos, André Michel

AU - de Souza, Daniel Martins

AU - de Lima Meirelles, Paulo Roberto

AU - Cru, Igor Vilela

AU - Moretti, Luiz Gustavo

AU - Bossolani, João William

AU - Kuramae, Eiko Eurya

N1 - Data archiving: no NIOO data

PY - 2025/6/4

Y1 - 2025/6/4

N2 - Background: Intercropping systems enhance agricultural sustainability by promoting ecosystem multifunctionality (EMF). This study examined the impact of adding pigeon pea (M + PG + PP) into a maize–palisade grass (M + PG) intercropping system under a no-till system (NTS) on soil microbial communities and ecosystem services. After five consecutive growing seasons, bulk soil samples from a soybean-based crop-livestock system were analyzed using metagenomics. Results: The inclusion of pigeon pea significantly improved the EMF index, with higher plant productivity and slightly enhanced outcomes in soil health, lamb meat productivity, and climate protection. The M + PG + PP treatment enriched Bradyrhizobium spp., which were positively correlated with soil health, plant productivity, and EMF index. Functional analysis indicated that M + PG + PP treatment enhanced nitrogen metabolism, biofilm formation, and exopolysaccharide (EPS) biosynthesis, improving soil fertility and microbial activity. Similarly, functional analysis of microbial plant growth-promoting traits revealed that the M + PG + PP treatment promoted microbial functions related to nitrogen and iron acquisition, sulfur assimilation, and plant colonization, all essential for plant growth and nutrient cycling. In contrast, the M + PG treatment primarily enhanced pathways related to competitive exclusion and phytohormone production. Conclusions: These findings highlight the importance of incorporating legumes such as pigeon pea into intercropping systems to optimize ecosystem services, enhance soil health, and promote long-term agricultural productivity and sustainability.

AB - Background: Intercropping systems enhance agricultural sustainability by promoting ecosystem multifunctionality (EMF). This study examined the impact of adding pigeon pea (M + PG + PP) into a maize–palisade grass (M + PG) intercropping system under a no-till system (NTS) on soil microbial communities and ecosystem services. After five consecutive growing seasons, bulk soil samples from a soybean-based crop-livestock system were analyzed using metagenomics. Results: The inclusion of pigeon pea significantly improved the EMF index, with higher plant productivity and slightly enhanced outcomes in soil health, lamb meat productivity, and climate protection. The M + PG + PP treatment enriched Bradyrhizobium spp., which were positively correlated with soil health, plant productivity, and EMF index. Functional analysis indicated that M + PG + PP treatment enhanced nitrogen metabolism, biofilm formation, and exopolysaccharide (EPS) biosynthesis, improving soil fertility and microbial activity. Similarly, functional analysis of microbial plant growth-promoting traits revealed that the M + PG + PP treatment promoted microbial functions related to nitrogen and iron acquisition, sulfur assimilation, and plant colonization, all essential for plant growth and nutrient cycling. In contrast, the M + PG treatment primarily enhanced pathways related to competitive exclusion and phytohormone production. Conclusions: These findings highlight the importance of incorporating legumes such as pigeon pea into intercropping systems to optimize ecosystem services, enhance soil health, and promote long-term agricultural productivity and sustainability.

KW - Cajanus cajan

KW - Crop-livestock integration

KW - legume-Bradyrhizobium interaction

KW - Shotgun metagenomics

KW - Soil fertility

KW - Sustainable agriculture

U2 - 10.1186/s40793-025-00727-0

DO - 10.1186/s40793-025-00727-0

M3 - Article

AN - SCOPUS:105007233896

SN - 2524-6372

VL - 20

JO - Environmental Microbiome

JF - Environmental Microbiome

M1 - 60

ER -