Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.

P.P. Das; M.P. Bagijn; L.D. Goldstein; J.R. Woolford; N.J. Lehrbach; A. Sapetschnig; H.R. Buhecha; M.J. Gilchrist; K.L Howe; R. Stark; N. Matthews; E. Berezikov; R.F. Ketting; S. Tavare; E.A. Miska

doi:10.1016/j.molcel.2008.06.003

Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.

P.P. Das, M.P. Bagijn, L.D. Goldstein, J.R. Woolford, N.J. Lehrbach, A. Sapetschnig, H.R. Buhecha, M.J. Gilchrist, K.L Howe, R. Stark, N. Matthews, E. Berezikov, R.F. Ketting, S. Tavare, E.A. Miska

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

321 Citations (Scopus)

Abstract

The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.

Original language	English
Pages (from-to)	79-90
Journal	Molecular Cell
Volume	31
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2008.06.003
Publication status	Published - 2008

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Das, P. P., Bagijn, M. P., Goldstein, L. D., Woolford, J. R., Lehrbach, N. J., Sapetschnig, A., Buhecha, H. R., Gilchrist, M. J., Howe, K. L., Stark, R., Matthews, N., Berezikov, E., Ketting, R. F., Tavare, S., & Miska, E. A. (2008). Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline. Molecular Cell, 31(1), 79-90. https://doi.org/10.1016/j.molcel.2008.06.003

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title = "Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.",

abstract = "The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.",

author = "P.P. Das and M.P. Bagijn and L.D. Goldstein and J.R. Woolford and N.J. Lehrbach and A. Sapetschnig and H.R. Buhecha and M.J. Gilchrist and K.L Howe and R. Stark and N. Matthews and E. Berezikov and R.F. Ketting and S. Tavare and E.A. Miska",

note = "Reporting year: 2008",

year = "2008",

doi = "10.1016/j.molcel.2008.06.003",

language = "English",

volume = "31",

pages = "79--90",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Cell Press",

number = "1",

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Das, PP, Bagijn, MP, Goldstein, LD, Woolford, JR, Lehrbach, NJ, Sapetschnig, A, Buhecha, HR, Gilchrist, MJ, Howe, KL, Stark, R, Matthews, N, Berezikov, E, Ketting, RF, Tavare, S & Miska, EA 2008, 'Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.', Molecular Cell, vol. 31, no. 1, pp. 79-90. https://doi.org/10.1016/j.molcel.2008.06.003

TY - JOUR

T1 - Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.

AU - Das, P.P.

AU - Bagijn, M.P.

AU - Goldstein, L.D.

AU - Woolford, J.R.

AU - Lehrbach, N.J.

AU - Sapetschnig, A.

AU - Buhecha, H.R.

AU - Gilchrist, M.J.

AU - Howe, K.L

AU - Stark, R.

AU - Matthews, N.

AU - Berezikov, E.

AU - Ketting, R.F.

AU - Tavare, S.

AU - Miska, E.A.

N1 - Reporting year: 2008

PY - 2008

Y1 - 2008

N2 - The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.

AB - The Piwi proteins of the Argonaute superfamily are required for normal germline development in Drosophila, zebrafish, and mice and associate with 24-30 nucleotide RNAs termed piRNAs. We identify a class of 21 nucleotide RNAs, previously named 21U-RNAs, as the piRNAs of C. elegans. Piwi and piRNA expression is restricted to the male and female germline and independent of many proteins in other small-RNA pathways, including DCR-1. We show that Piwi is specifically required to silence Tc3, but not other Tc/mariner DNA transposons. Tc3 excision rates in the germline are increased at least 100-fold in piwi mutants as compared to wild-type. We find no evidence for a Ping-Pong model for piRNA amplification in C. elegans. Instead, we demonstrate that Piwi acts upstream of an endogenous siRNA pathway in Tc3 silencing. These data might suggest a link between piRNA and siRNA function.

U2 - 10.1016/j.molcel.2008.06.003

DO - 10.1016/j.molcel.2008.06.003

M3 - Article

SN - 1097-2765

VL - 31

SP - 79

EP - 90

JO - Molecular Cell

JF - Molecular Cell

IS - 1

ER -

Piwi and piRNAs act upstream of an endogenous siRNA pathway to suppress Tc3 transposon mobility in the Caenorhabditis elegans germline.

Abstract

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