Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos

Roel Neijts; Shilu Amin; Carina van Rooijen; Sander Tan; Menno P Creyghton; Wouter de Laat; Jacqueline Deschamps

doi:10.1101/gad.285767.116

Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos

Roel Neijts, Shilu Amin, Carina van Rooijen, Sander Tan, Menno P Creyghton, Wouter de Laat, Jacqueline Deschamps

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

72 Citations (Scopus)

Abstract

Sequential 3'-to-5' activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3'-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3' subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3'-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3' side of the cluster.

Original language	English
Pages (from-to)	1937-42
Number of pages	6
Journal	Genes & Development
Volume	30
Issue number	17
DOIs	https://doi.org/10.1101/gad.285767.116
Publication status	Published - 01 Sept 2016

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10.1101/gad.285767.116

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abstract = "Sequential 3'-to-5' activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3'-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3' subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3'-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3' side of the cluster.",

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AU - Neijts, Roel

AU - Amin, Shilu

AU - van Rooijen, Carina

AU - Tan, Sander

AU - Creyghton, Menno P

AU - de Laat, Wouter

AU - Deschamps, Jacqueline

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N2 - Sequential 3'-to-5' activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3'-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3' subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3'-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3' side of the cluster.

AB - Sequential 3'-to-5' activation of the Hox gene clusters in early embryos is a most fascinating issue in developmental biology. Neither the trigger nor the regulatory elements involved in the transcriptional initiation of the 3'-most Hox genes have been unraveled in any organism. We demonstrate that a series of enhancers, some of which are Wnt-dependent, is located within a HoxA 3' subtopologically associated domain (subTAD). This subTAD forms the structural basis for multiple layers of 3'-polarized features, including DNA accessibility and enhancer activation. Deletion of the cassette of Wnt-dependent enhancers proves its crucial role in initial transcription of HoxA at the 3' side of the cluster.

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VL - 30

SP - 1937

EP - 1942

JO - Genes & Development

JF - Genes & Development

IS - 17

ER -

Polarized regulatory landscape and Wnt responsiveness underlie Hox activation in embryos

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