Potassium phosphite enhanced the suppressive capacity of the soil microbiome against the tomato pathogen Ralstonia solanacearum

Lv Su; Haichao Feng; Xingxia Mo; Juan Sun; Pengfei Qiu; Yunpeng Liu; Ruifu Zhang; Eiko Kuramae; Biao Shen; Qirong Shen

doi:10.1007/s00374-022-01634-z

Potassium phosphite enhanced the suppressive capacity of the soil microbiome against the tomato pathogen Ralstonia solanacearum

Lv Su (Corresponding author), Haichao Feng, Xingxia Mo, Juan Sun, Pengfei Qiu, Yunpeng Liu, Ruifu Zhang, Eiko Kuramae, Biao Shen^* (Corresponding author), Qirong Shen

^*Corresponding author for this work

NIOO - Microbial Ecology (ME)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

4 Citations (Scopus)

55 Downloads (Pure)

Abstract

High-throughput sequencing, culture-dependent workflows, and microbiome transfer experiments reveal whether potassium phosphite (KP), an environmentally acceptable agricultural chemical, could specifically enrich the antagonistic bacterial community that inhibited the growth of the pathogen Ralstonia solanacearum. The application of KP enriched the potential antagonistic bacteria Paenibacillus and Streptomyces in soil, but depleted most dominant genera belonging to gram negative bacteria, such as Pseudomonas, Massilia, and Flavobacterium on day 7. Moreover, the KP-modulated soil microbiome suppressed R. solanacearum growth in soil. The predicted functions related to the synthesis of antagonistic substances, such as streptomycin, and the predicted functions related to tellurite resistance and nickel transport system were significantly enriched, but the synthesis of lipopolysaccharide (distinct component lipopolysaccharide in gram negative bacteria) were significantly depleted in the KP-treated soils. In addition, the copy numbers of specific sequences for Streptomyces coelicoflavus and Paenibacillus favisporus were significantly increased in the soil amended with KP, inhibited the growth of R. solanacearum, and had a higher tolerance of KP than R. solanacearum. Our study linked the application of fertilizers to the enrichment of antagonistic bacteria, which could support future work that aims to precisely regulate the soil microbiome to protect the host from infection by soil-borne pathogens.

Original language	English
Pages (from-to)	553–563
Number of pages	11
Journal	Biology and Fertility of Soils
Volume	58
Issue number	5
DOIs	https://doi.org/10.1007/s00374-022-01634-z
Publication status	Published - 2022

Keywords

Agrochemical
Antagonistic strains
Soil bacterial community
Soil-borne pathogen

Access to Document

10.1007/s00374-022-01634-z

Su et al 2022Final published version, 1.34 MB

Cite this

@article{b52a51f8edb14e1db9a3ee6a7927cb7d,

title = "Potassium phosphite enhanced the suppressive capacity of the soil microbiome against the tomato pathogen Ralstonia solanacearum",

abstract = "High-throughput sequencing, culture-dependent workflows, and microbiome transfer experiments reveal whether potassium phosphite (KP), an environmentally acceptable agricultural chemical, could specifically enrich the antagonistic bacterial community that inhibited the growth of the pathogen Ralstonia solanacearum. The application of KP enriched the potential antagonistic bacteria Paenibacillus and Streptomyces in soil, but depleted most dominant genera belonging to gram negative bacteria, such as Pseudomonas, Massilia, and Flavobacterium on day 7. Moreover, the KP-modulated soil microbiome suppressed R. solanacearum growth in soil. The predicted functions related to the synthesis of antagonistic substances, such as streptomycin, and the predicted functions related to tellurite resistance and nickel transport system were significantly enriched, but the synthesis of lipopolysaccharide (distinct component lipopolysaccharide in gram negative bacteria) were significantly depleted in the KP-treated soils. In addition, the copy numbers of specific sequences for Streptomyces coelicoflavus and Paenibacillus favisporus were significantly increased in the soil amended with KP, inhibited the growth of R. solanacearum, and had a higher tolerance of KP than R. solanacearum. Our study linked the application of fertilizers to the enrichment of antagonistic bacteria, which could support future work that aims to precisely regulate the soil microbiome to protect the host from infection by soil-borne pathogens.",

keywords = "Agrochemical, Antagonistic strains, Soil bacterial community, Soil-borne pathogen",

author = "Lv Su and Haichao Feng and Xingxia Mo and Juan Sun and Pengfei Qiu and Yunpeng Liu and Ruifu Zhang and Eiko Kuramae and Biao Shen and Qirong Shen",

note = "7417, ME; Data: Deposited NCBI Sequence Read Archive (SRA) accession PRJNA577427",

year = "2022",

doi = "10.1007/s00374-022-01634-z",

language = "English",

volume = "58",

pages = "553–563",

journal = "Biology and Fertility of Soils",

issn = "0178-2762",

publisher = "Springer Verlag GmbH",

number = "5",

}

TY - JOUR

T1 - Potassium phosphite enhanced the suppressive capacity of the soil microbiome against the tomato pathogen Ralstonia solanacearum

AU - Su, Lv

AU - Feng, Haichao

AU - Mo, Xingxia

AU - Sun, Juan

AU - Qiu, Pengfei

AU - Liu, Yunpeng

AU - Zhang, Ruifu

AU - Kuramae, Eiko

AU - Shen, Biao

AU - Shen, Qirong

N1 - 7417, ME; Data: Deposited NCBI Sequence Read Archive (SRA) accession PRJNA577427

PY - 2022

Y1 - 2022

N2 - High-throughput sequencing, culture-dependent workflows, and microbiome transfer experiments reveal whether potassium phosphite (KP), an environmentally acceptable agricultural chemical, could specifically enrich the antagonistic bacterial community that inhibited the growth of the pathogen Ralstonia solanacearum. The application of KP enriched the potential antagonistic bacteria Paenibacillus and Streptomyces in soil, but depleted most dominant genera belonging to gram negative bacteria, such as Pseudomonas, Massilia, and Flavobacterium on day 7. Moreover, the KP-modulated soil microbiome suppressed R. solanacearum growth in soil. The predicted functions related to the synthesis of antagonistic substances, such as streptomycin, and the predicted functions related to tellurite resistance and nickel transport system were significantly enriched, but the synthesis of lipopolysaccharide (distinct component lipopolysaccharide in gram negative bacteria) were significantly depleted in the KP-treated soils. In addition, the copy numbers of specific sequences for Streptomyces coelicoflavus and Paenibacillus favisporus were significantly increased in the soil amended with KP, inhibited the growth of R. solanacearum, and had a higher tolerance of KP than R. solanacearum. Our study linked the application of fertilizers to the enrichment of antagonistic bacteria, which could support future work that aims to precisely regulate the soil microbiome to protect the host from infection by soil-borne pathogens.

AB - High-throughput sequencing, culture-dependent workflows, and microbiome transfer experiments reveal whether potassium phosphite (KP), an environmentally acceptable agricultural chemical, could specifically enrich the antagonistic bacterial community that inhibited the growth of the pathogen Ralstonia solanacearum. The application of KP enriched the potential antagonistic bacteria Paenibacillus and Streptomyces in soil, but depleted most dominant genera belonging to gram negative bacteria, such as Pseudomonas, Massilia, and Flavobacterium on day 7. Moreover, the KP-modulated soil microbiome suppressed R. solanacearum growth in soil. The predicted functions related to the synthesis of antagonistic substances, such as streptomycin, and the predicted functions related to tellurite resistance and nickel transport system were significantly enriched, but the synthesis of lipopolysaccharide (distinct component lipopolysaccharide in gram negative bacteria) were significantly depleted in the KP-treated soils. In addition, the copy numbers of specific sequences for Streptomyces coelicoflavus and Paenibacillus favisporus were significantly increased in the soil amended with KP, inhibited the growth of R. solanacearum, and had a higher tolerance of KP than R. solanacearum. Our study linked the application of fertilizers to the enrichment of antagonistic bacteria, which could support future work that aims to precisely regulate the soil microbiome to protect the host from infection by soil-borne pathogens.

KW - Agrochemical

KW - Antagonistic strains

KW - Soil bacterial community

KW - Soil-borne pathogen

U2 - 10.1007/s00374-022-01634-z

DO - 10.1007/s00374-022-01634-z

M3 - Article

SN - 0178-2762

VL - 58

SP - 553

EP - 563

JO - Biology and Fertility of Soils

JF - Biology and Fertility of Soils

IS - 5

ER -

Potassium phosphite enhanced the suppressive capacity of the soil microbiome against the tomato pathogen Ralstonia solanacearum

Abstract

Keywords

Access to Document

Fingerprint

Cite this