Premigratory and migratory neural crest cells are multipotent in vivo

TY - JOUR

T1 - Premigratory and migratory neural crest cells are multipotent in vivo

AU - Baggiolini, Arianna

AU - Varum, Sandra

AU - Mateos, José María

AU - Bettosini, Damiano

AU - John, Nessy

AU - Bonalli, Mario

AU - Ziegler, Urs

AU - Dimou, Leda

AU - Clevers, Hans

AU - Furrer, Reinhard

AU - Sommer, Lukas

N1 - Copyright © 2015 Elsevier Inc. All rights reserved.

PY - 2015/3/5

Y1 - 2015/3/5

N2 - The neural crest (NC) is an embryonic stem/progenitor cell population that generates a diverse array of cell lineages, including peripheral neurons, myelinating Schwann cells, and melanocytes, among others. However, there is a long-standing controversy as to whether this broad developmental perspective reflects in vivo multipotency of individual NC cells or whether the NC is comprised of a heterogeneous mixture of lineage-restricted progenitors. Here, we resolve this controversy by performing in vivo fate mapping of single trunk NC cells both at premigratory and migratory stages using the R26R-Confetti mouse model. By combining quantitative clonal analyses with definitive markers of differentiation, we demonstrate that the vast majority of individual NC cells are multipotent, with only few clones contributing to single derivatives. Intriguingly, multipotency is maintained in migratory NC cells. Thus, our findings provide definitive evidence for the in vivo multipotency of both premigratory and migrating NC cells in the mouse.

AB - The neural crest (NC) is an embryonic stem/progenitor cell population that generates a diverse array of cell lineages, including peripheral neurons, myelinating Schwann cells, and melanocytes, among others. However, there is a long-standing controversy as to whether this broad developmental perspective reflects in vivo multipotency of individual NC cells or whether the NC is comprised of a heterogeneous mixture of lineage-restricted progenitors. Here, we resolve this controversy by performing in vivo fate mapping of single trunk NC cells both at premigratory and migratory stages using the R26R-Confetti mouse model. By combining quantitative clonal analyses with definitive markers of differentiation, we demonstrate that the vast majority of individual NC cells are multipotent, with only few clones contributing to single derivatives. Intriguingly, multipotency is maintained in migratory NC cells. Thus, our findings provide definitive evidence for the in vivo multipotency of both premigratory and migrating NC cells in the mouse.

U2 - 10.1016/j.stem.2015.02.017

DO - 10.1016/j.stem.2015.02.017

M3 - Article

C2 - 25748934

SN - 1934-5909

VL - 16

SP - 314

EP - 322

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 3

ER -