Prenatal NeuN+ neurons of Down syndrome display aberrant integrative DNA methylation and gene expression profiles

Peter Henneman; Adri N Mul; Andrew Yf Li Yim; Izabela M Krzyzewska; Mariëlle Alders; Adelia Adelia; Mark R Mizee; Marcel M Mannens

doi:10.2217/epi-2021-0523

Prenatal NeuN+ neurons of Down syndrome display aberrant integrative DNA methylation and gene expression profiles

Peter Henneman, Adri N Mul, Andrew Yf Li Yim, Izabela M Krzyzewska, Mariëlle Alders, Adelia Adelia, Mark R Mizee, Marcel M Mannens

Netherlands Institute for Neuroscience (NIN)

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Abstract

Aim: To detect expression quantitative trait methylation (eQTM) loci within the cerebrum of prenatal Down syndrome (DS) and controls. Material & methods: DNA methylation gene expression profiles were acquired from NeuN+ nuclei, obtained from cerebrum sections of DS and controls. Linear regression models were applied to both datasets and were subsequently applied in an integrative analysis model to detect DS-associated eQTM loci. Results & conclusion: Widespread aberrant DNA methylation and gene expression were observed in DS. A substantial number of differentially methylated loci were replicated according to a previously reported study. Subsequent integrative analyses (eQTM) yielded numerous associated DS loci. the authors associated DNA methylation, gene expression and eQTM loci with DS that may underlie particular DS phenotypical characteristics.

Original language	English
Pages (from-to)	375-390
Journal	Epigenomics
Volume	14
DOIs	https://doi.org/10.2217/epi-2021-0523
Publication status	Published - 02 Mar 2022

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title = "Prenatal NeuN+ neurons of Down syndrome display aberrant integrative DNA methylation and gene expression profiles",

abstract = "Aim: To detect expression quantitative trait methylation (eQTM) loci within the cerebrum of prenatal Down syndrome (DS) and controls. Material & methods: DNA methylation gene expression profiles were acquired from NeuN+ nuclei, obtained from cerebrum sections of DS and controls. Linear regression models were applied to both datasets and were subsequently applied in an integrative analysis model to detect DS-associated eQTM loci. Results & conclusion: Widespread aberrant DNA methylation and gene expression were observed in DS. A substantial number of differentially methylated loci were replicated according to a previously reported study. Subsequent integrative analyses (eQTM) yielded numerous associated DS loci. the authors associated DNA methylation, gene expression and eQTM loci with DS that may underlie particular DS phenotypical characteristics.",

author = "Peter Henneman and Mul, {Adri N} and {Li Yim}, {Andrew Yf} and Krzyzewska, {Izabela M} and Mari{\"e}lle Alders and Adelia Adelia and Mizee, {Mark R} and Mannens, {Marcel M}",

year = "2022",

month = mar,

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doi = "10.2217/epi-2021-0523",

language = "English",

volume = "14",

pages = "375--390",

journal = "Epigenomics",

issn = "1750-192X",

publisher = "Future Medicine Ltd.",

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TY - JOUR

T1 - Prenatal NeuN+ neurons of Down syndrome display aberrant integrative DNA methylation and gene expression profiles

AU - Henneman, Peter

AU - Mul, Adri N

AU - Li Yim, Andrew Yf

AU - Krzyzewska, Izabela M

AU - Alders, Mariëlle

AU - Adelia, Adelia

AU - Mizee, Mark R

AU - Mannens, Marcel M

PY - 2022/3/2

Y1 - 2022/3/2

N2 - Aim: To detect expression quantitative trait methylation (eQTM) loci within the cerebrum of prenatal Down syndrome (DS) and controls. Material & methods: DNA methylation gene expression profiles were acquired from NeuN+ nuclei, obtained from cerebrum sections of DS and controls. Linear regression models were applied to both datasets and were subsequently applied in an integrative analysis model to detect DS-associated eQTM loci. Results & conclusion: Widespread aberrant DNA methylation and gene expression were observed in DS. A substantial number of differentially methylated loci were replicated according to a previously reported study. Subsequent integrative analyses (eQTM) yielded numerous associated DS loci. the authors associated DNA methylation, gene expression and eQTM loci with DS that may underlie particular DS phenotypical characteristics.

AB - Aim: To detect expression quantitative trait methylation (eQTM) loci within the cerebrum of prenatal Down syndrome (DS) and controls. Material & methods: DNA methylation gene expression profiles were acquired from NeuN+ nuclei, obtained from cerebrum sections of DS and controls. Linear regression models were applied to both datasets and were subsequently applied in an integrative analysis model to detect DS-associated eQTM loci. Results & conclusion: Widespread aberrant DNA methylation and gene expression were observed in DS. A substantial number of differentially methylated loci were replicated according to a previously reported study. Subsequent integrative analyses (eQTM) yielded numerous associated DS loci. the authors associated DNA methylation, gene expression and eQTM loci with DS that may underlie particular DS phenotypical characteristics.

U2 - 10.2217/epi-2021-0523

DO - 10.2217/epi-2021-0523

M3 - Article

C2 - 35232286

SN - 1750-192X

VL - 14

SP - 375

EP - 390

JO - Epigenomics

JF - Epigenomics

ER -

Prenatal NeuN+ neurons of Down syndrome display aberrant integrative DNA methylation and gene expression profiles

Abstract

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