Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

Je-Seung Jeon; Ji Hoon Kim; Chae Lee Park; Chul Young Kim

doi:10.1080/10826076.2015.1091010

Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

Je-Seung Jeon, Ji Hoon Kim, Chae Lee Park, Chul Young Kim

NIOO - Microbial Ecology (ME)

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

4 Citations (Scopus)

396 Downloads (Pure)

Abstract

Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH•-HPLC experiment. In DPPH•-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3 mg), dihydrokaempferol 7-O-β-D-glucopyranoside (230.4 mg), and trans-piceid (165.9 mg) were purified from 2.19 g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by 1H, 13C NMR, and ESI-MS.

Original language	English
Pages (from-to)	1681-1686
Journal	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume	38
Issue number	18
DOIs	https://doi.org/10.1080/10826076.2015.1091010
Publication status	Published - 08 Nov 2015

Keywords

Abies koreana Wilson
centrifugal partition chromatography
DPPH center dot-HPLC experiment
dihydrokaempferol 7-O--D-glucopyranoside
maltol
trans-piceid
international

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5982_JeonFinal published version, 1.17 MB

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@article{e06b8c7ed743485786fe5c81864a6c7d,

title = "Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment",

abstract = "Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH•-HPLC experiment. In DPPH•-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3 mg), dihydrokaempferol 7-O-β-D-glucopyranoside (230.4 mg), and trans-piceid (165.9 mg) were purified from 2.19 g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by 1H, 13C NMR, and ESI-MS.",

keywords = "Abies koreana Wilson, centrifugal partition chromatography, DPPH center dot-HPLC experiment, dihydrokaempferol 7-O--D-glucopyranoside, maltol, trans-piceid, international",

author = "Je-Seung Jeon and Kim, {Ji Hoon} and Park, {Chae Lee} and Kim, {Chul Young}",

note = "5982, ME; Data archiving: Property of and archived at Hanyang University (Korea)",

year = "2015",

month = nov,

day = "8",

doi = "10.1080/10826076.2015.1091010",

language = "English",

volume = "38",

pages = "1681--1686",

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issn = "1082-6076",

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Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment. / Jeon, Je-Seung; Kim, Ji Hoon; Park, Chae Lee et al.
In: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Vol. 38, No. 18, 08.11.2015, p. 1681-1686.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

AU - Jeon, Je-Seung

AU - Kim, Ji Hoon

AU - Park, Chae Lee

AU - Kim, Chul Young

N1 - 5982, ME; Data archiving: Property of and archived at Hanyang University (Korea)

PY - 2015/11/8

Y1 - 2015/11/8

N2 - Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH•-HPLC experiment. In DPPH•-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3 mg), dihydrokaempferol 7-O-β-D-glucopyranoside (230.4 mg), and trans-piceid (165.9 mg) were purified from 2.19 g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by 1H, 13C NMR, and ESI-MS.

AB - Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH•-HPLC experiment. In DPPH•-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3 mg), dihydrokaempferol 7-O-β-D-glucopyranoside (230.4 mg), and trans-piceid (165.9 mg) were purified from 2.19 g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by 1H, 13C NMR, and ESI-MS.

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KW - dihydrokaempferol 7-O--D-glucopyranoside

KW - maltol

KW - trans-piceid

KW - international

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M3 - Article

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VL - 38

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JO - JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

JF - JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

IS - 18

ER -

Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

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Keywords

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