Preparative Isolation of Polar Antioxidant Constituents from Abies koreana Using Centrifugal Partition Chromatography Guided by DPPH center dot-HPLC Experiment

Je-Seung Jeon, Ji Hoon Kim, Chae Lee Park, Chul Young Kim

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Abstract

Preparative separation of antioxidant constituents from the leaves of Abies koreana Wilson (Pinaceae) was performed by centrifugal partition chromatography (CPC) with a two-phase solvent system of ethyl acetate-isopropanol-water (9:1:10, v/v) target-guided by DPPH•-HPLC experiment. In DPPH•-HPLC experiment, trans-piceid exerted antioxidant activity, which was purified by Diaion-HP 20 macroporous resin chromatography and subsequent CPC. Maltol (98.3 mg), dihydrokaempferol 7-O-β-D-glucopyranoside (230.4 mg), and trans-piceid (165.9 mg) were purified from 2.19 g of crude extract with the purity over 95% by HPLC analysis. The chemical structures of isolated compounds were determined by 1H, 13C NMR, and ESI-MS.
Original languageEnglish
Pages (from-to)1681-1686
JournalJOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume38
Issue number18
DOIs
Publication statusPublished - 08 Nov 2015

Keywords

  • Abies koreana Wilson
  • centrifugal partition chromatography
  • DPPH center dot-HPLC experiment
  • dihydrokaempferol 7-O--D-glucopyranoside
  • maltol
  • trans-piceid
  • international

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