Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen, M. Aguadé, D. Charlesworth, A.D.E. Haan, B. Lauga, T. Mitchell-Olds, S. Oikarinen, S. Ramos-Onsins, B. Stranger, P.H. Van Tienderen, O. Savolainen

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    Abstract

    There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci. [KEYWORDS: Arabidopsis; candidate genes; defence-genes; flowering time; molecular adaptation; phenylpropanoid pathway; phytochromes]
    Original languageEnglish
    Pages (from-to)258-262
    JournalMolecular Ecology Notes
    Volume2
    Issue number3
    DOIs
    Publication statusPublished - 2002

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