Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen; M. Aguadé; D. Charlesworth; A.D.E. Haan; B. Lauga; T. Mitchell-Olds; S. Oikarinen; S. Ramos-Onsins; B. Stranger; P.H. Van Tienderen; O. Savolainen

doi:10.1046/j.1471-8286.2002.00210.x

Primers for 22 candidate genes for ecological adaptations in Brassicaceae

H. Kuittinen, M. Aguadé, D. Charlesworth, A.D.E. Haan, B. Lauga, T. Mitchell-Olds, S. Oikarinen, S. Ramos-Onsins, B. Stranger, P.H. Van Tienderen, O. Savolainen

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Abstract

There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci. [KEYWORDS: Arabidopsis; candidate genes; defence-genes; flowering time; molecular adaptation; phenylpropanoid pathway; phytochromes]

Original language	English
Pages (from-to)	258-262
Journal	Molecular Ecology Notes
Volume	2
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8286.2002.00210.x
Publication status	Published - 2002

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title = "Primers for 22 candidate genes for ecological adaptations in Brassicaceae",

abstract = "There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci. [KEYWORDS: Arabidopsis; candidate genes; defence-genes; flowering time; molecular adaptation; phenylpropanoid pathway; phytochromes]",

author = "H. Kuittinen and M. Aguad{\'e} and D. Charlesworth and A.D.E. Haan and B. Lauga and T. Mitchell-Olds and S. Oikarinen and S. Ramos-Onsins and B. Stranger and {Van Tienderen}, P.H. and O. Savolainen",

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doi = "10.1046/j.1471-8286.2002.00210.x",

language = "English",

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T1 - Primers for 22 candidate genes for ecological adaptations in Brassicaceae

AU - Kuittinen, H.

AU - Aguadé, M.

AU - Charlesworth, D.

AU - Haan, A.D.E.

AU - Lauga, B.

AU - Mitchell-Olds, T.

AU - Oikarinen, S.

AU - Ramos-Onsins, S.

AU - Stranger, B.

AU - Van Tienderen, P.H.

AU - Savolainen, O.

N1 - Reporting year: 2002 Metis note: 3069; CTE; PVP; file:///L:/Endnotedatabases/NIOOPUB/pdfs/Pdfs2002/Kuittien_ea_3069.pdf

PY - 2002

Y1 - 2002

N2 - There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci. [KEYWORDS: Arabidopsis; candidate genes; defence-genes; flowering time; molecular adaptation; phenylpropanoid pathway; phytochromes]

AB - There is an increasing interest in direct screening of polymorphisms at candidate loci to associate them with adaptations in natural situations. We report primers that amplify regions at 22 putatively orthologous functional loci in the family Brassicaceae: Arabidopsis thaliana, its two wild outcrossing relatives, and Brassica oleracea. Four groups of genes were known to have specific functions in the model species A. thaliana in ecologically important traits: timing of reproduction; metabolism of secondary compounds; defence against pathogens or light perception. In addition, genes of a fifth group were selected with no consideration of their function, as reference loci. [KEYWORDS: Arabidopsis; candidate genes; defence-genes; flowering time; molecular adaptation; phenylpropanoid pathway; phytochromes]

U2 - 10.1046/j.1471-8286.2002.00210.x

DO - 10.1046/j.1471-8286.2002.00210.x

M3 - Article

SN - 1471-8278

VL - 2

SP - 258

EP - 262

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Primers for 22 candidate genes for ecological adaptations in Brassicaceae

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