Production of iPS-Derived Human Retinal Organoids for Use in Transgene Expression Assays

Peter M Quinn, Thilo M Buck, Charlotte Ohonin, Harald M M Mikkers, J. Wijnholds

Research output: Contribution to journal/periodicalArticleScientificpeer-review

Abstract

In vitro retinal organoid modeling from human pluripotent stem cells is becoming more common place in many ophthalmic laboratories worldwide. These organoids mimic human retinogenesis through formation of organized layered retinal structures that display markers for typical retinal cell types. Pivotally these humanized retinal models provide a stepping stone to the clinic as therapeutic tools and are expected to provide a promising alternative to current animal models. Thus pluripotent stem cell based healthy as well as diseased human retinal organoids are attractive for use in drug potency assays and gene augmentation therapeutics. Here we outline an established protocol for generation of these retinal organoids and how they can be used in conjunction with adeno-associated virus vectors for transgene expression assays.

Original languageEnglish
Pages (from-to)261-273
Number of pages13
JournalMethods in Molecular Biology
Volume1715
DOIs
Publication statusPublished - 2018

Keywords

  • Journal Article

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