Protein Tyrosine Phosphatase Studies in Zebrafish

Daniëlle T J Woutersen; Jisca Majolée; Jeroen den Hertog

doi:10.1007/978-1-0716-3569-8_6

Protein Tyrosine Phosphatase Studies in Zebrafish

Daniëlle T J Woutersen, Jisca Majolée, Jeroen den Hertog

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

1 Citation (Scopus)

Abstract

The zebrafish is an ideal model for functional analysis of genes at the molecular, protein, cell, organ, and organism levels. We have used zebrafish to analyze the function of members of the protein tyrosine phosphatase (PTP) superfamily for more than two decades. The molecular genetic toolbox has significantly improved over the years. Currently, generating mutant lines that lack the function of a PTP gene is relatively straightforward by CRISPR/Cas9 technology-mediated generation of insertions or deletions in the target gene. In addition, generating point mutations using CRISPR/Cas9 technology and homology-directed repair (HDR) is feasible, albeit the success rate could be higher. Here, we describe the methods, including the tips and tricks, that we have used to generate knock-out and knock-in zebrafish lines in PTP genes successfully.

Original language	English
Pages (from-to)	93-110
Number of pages	18
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2743
DOIs	https://doi.org/10.1007/978-1-0716-3569-8_6
Publication status	Published - 2024

Keywords

Animals
Zebrafish/genetics
Protein Tyrosine Phosphatases/genetics
Craniocerebral Trauma
Perciformes
Point Mutation

Access to Document

10.1007/978-1-0716-3569-8_6

Cite this

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title = "Protein Tyrosine Phosphatase Studies in Zebrafish",

abstract = "The zebrafish is an ideal model for functional analysis of genes at the molecular, protein, cell, organ, and organism levels. We have used zebrafish to analyze the function of members of the protein tyrosine phosphatase (PTP) superfamily for more than two decades. The molecular genetic toolbox has significantly improved over the years. Currently, generating mutant lines that lack the function of a PTP gene is relatively straightforward by CRISPR/Cas9 technology-mediated generation of insertions or deletions in the target gene. In addition, generating point mutations using CRISPR/Cas9 technology and homology-directed repair (HDR) is feasible, albeit the success rate could be higher. Here, we describe the methods, including the tips and tricks, that we have used to generate knock-out and knock-in zebrafish lines in PTP genes successfully.",

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KW - Craniocerebral Trauma

KW - Perciformes

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