Proteins Potentially Involved in Immune Evasion Strategies in Sporothrix brasiliensis Elucidated by Ultra-High-Resolution Mass Spectrometry

TY - JOUR

T1 - Proteins Potentially Involved in Immune Evasion Strategies in Sporothrix brasiliensis Elucidated by Ultra-High-Resolution Mass Spectrometry

AU - Rossato, Luana

AU - Moreno, Leandro Ferreira

AU - Jamalian, Azadeh

AU - Stielow, Benjamin

AU - de Almeida, Sandro Rogério

AU - de Hoog, Sybren

AU - Freeke, Joanna

A2 - Bahn, Yong-Sun

PY - 2018/6/13

Y1 - 2018/6/13

N2 - Sporothrix brasiliensis is the prevalent agent of a large zoonotic outbreak in Brazil. With the involvement of several thousands of cases, this is the largest cohort of human and animal sporotrichosis on record in the world. Infections are characterized by local cutaneous dissemination in humans without underlying disease. S. brasiliensis has shown a high degree of virulence in a mouse model compared to the remaining Sporothrix species, including the ancestral species, Sporothrix schenckii . The present paper investigates a genomic and expressed-proteome comparison of S. brasiliensis to S. schenckii . Using bottom-up proteomics, we found 60 proteins exclusively expressed in S. brasiliensis . No significant genomic differences were found among the genes coding for this protein set. A comparison with literature data identified nine proteins that are known to be involved in virulence and immune evasion in other species, several of which had not yet been reported for the Sporothrix species analyzed.

AB - Sporothrix brasiliensis is the prevalent agent of a large zoonotic outbreak in Brazil. With the involvement of several thousands of cases, this is the largest cohort of human and animal sporotrichosis on record in the world. Infections are characterized by local cutaneous dissemination in humans without underlying disease. S. brasiliensis has shown a high degree of virulence in a mouse model compared to the remaining Sporothrix species, including the ancestral species, Sporothrix schenckii . The present paper investigates a genomic and expressed-proteome comparison of S. brasiliensis to S. schenckii . Using bottom-up proteomics, we found 60 proteins exclusively expressed in S. brasiliensis . No significant genomic differences were found among the genes coding for this protein set. A comparison with literature data identified nine proteins that are known to be involved in virulence and immune evasion in other species, several of which had not yet been reported for the Sporothrix species analyzed.

U2 - 10.1128/mSphere.00514-17

DO - 10.1128/mSphere.00514-17

M3 - Article

C2 - 29898987

SN - 2379-5042

VL - 3

JO - mSphere

JF - mSphere

IS - 3

ER -