Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)

E. Decat; E. van Mechelen; B. Saerens; S.J.T. Vermeulen; T. Boekhout; S. de Blaiser; M. Vaneechoutte; P. Deschaght

doi:10.1016/j.resmic.2012.10.017

Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)

E. Decat, E. van Mechelen, B. Saerens, S.J.T. Vermeulen, T. Boekhout, S. de Blaiser, M. Vaneechoutte, P. Deschaght

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Abstract

Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishing between 16 Candida spp., i.e. Candida albicans, Candida bracarensis, Candida dubliniensis, Candida famata, Candida glabrata, Candida guilliermondii, Candida inconspicua, Candida kefyr, Candida krusei, Candida lipolytica, Candida lusitaniae, Candida nivariensis, Candida norvegensis, Candida parapsilosis, Candida tropicalis and Candida sojae, and Saccharomyces cerevisiae and one species pair, i.e. Candida metapsilosis/Candida orthopsilosis. Starting from a cultured isolate, ITS2-MCA led to differentiation of these species within 6 h. According to our findings, ITS2-MCA offers a simple, rapid and cost-effective method for identification of cultured isolates of the clinically most relevant and prevalent Candida species. Further studies will be necessary to evaluate how it performs on mixed samples and clinical samples.

Original language	English
Pages (from-to)	110-117
Journal	Research in Microbiology
Volume	164
Issue number	2
Early online date	06 Nov 2012
DOIs	https://doi.org/10.1016/j.resmic.2012.10.017
Publication status	Published - 2013

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10.1016/j.resmic.2012.10.017

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Decat, E., van Mechelen, E., Saerens, B., Vermeulen, S. J. T., Boekhout, T., de Blaiser, S., Vaneechoutte, M., & Deschaght, P. (2013). Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA). Research in Microbiology, 164(2), 110-117. https://doi.org/10.1016/j.resmic.2012.10.017

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title = "Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)",

abstract = "Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishing between 16 Candida spp., i.e. Candida albicans, Candida bracarensis, Candida dubliniensis, Candida famata, Candida glabrata, Candida guilliermondii, Candida inconspicua, Candida kefyr, Candida krusei, Candida lipolytica, Candida lusitaniae, Candida nivariensis, Candida norvegensis, Candida parapsilosis, Candida tropicalis and Candida sojae, and Saccharomyces cerevisiae and one species pair, i.e. Candida metapsilosis/Candida orthopsilosis. Starting from a cultured isolate, ITS2-MCA led to differentiation of these species within 6 h. According to our findings, ITS2-MCA offers a simple, rapid and cost-effective method for identification of cultured isolates of the clinically most relevant and prevalent Candida species. Further studies will be necessary to evaluate how it performs on mixed samples and clinical samples.",

author = "E. Decat and {van Mechelen}, E. and B. Saerens and S.J.T. Vermeulen and T. Boekhout and {de Blaiser}, S. and M. Vaneechoutte and P. Deschaght",

note = "Reporting year: 2012",

year = "2013",

doi = "10.1016/j.resmic.2012.10.017",

language = "English",

volume = "164",

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journal = "Research in Microbiology",

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Decat, E, van Mechelen, E, Saerens, B, Vermeulen, SJT, Boekhout, T, de Blaiser, S, Vaneechoutte, M & Deschaght, P 2013, 'Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)', Research in Microbiology, vol. 164, no. 2, pp. 110-117. https://doi.org/10.1016/j.resmic.2012.10.017

Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA). / Decat, E.; van Mechelen, E.; Saerens, B. et al.
In: Research in Microbiology, Vol. 164, No. 2, 2013, p. 110-117.

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

TY - JOUR

T1 - Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)

AU - Decat, E.

AU - van Mechelen, E.

AU - Saerens, B.

AU - Vermeulen, S.J.T.

AU - Boekhout, T.

AU - de Blaiser, S.

AU - Vaneechoutte, M.

AU - Deschaght, P.

N1 - Reporting year: 2012

PY - 2013

Y1 - 2013

N2 - Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishing between 16 Candida spp., i.e. Candida albicans, Candida bracarensis, Candida dubliniensis, Candida famata, Candida glabrata, Candida guilliermondii, Candida inconspicua, Candida kefyr, Candida krusei, Candida lipolytica, Candida lusitaniae, Candida nivariensis, Candida norvegensis, Candida parapsilosis, Candida tropicalis and Candida sojae, and Saccharomyces cerevisiae and one species pair, i.e. Candida metapsilosis/Candida orthopsilosis. Starting from a cultured isolate, ITS2-MCA led to differentiation of these species within 6 h. According to our findings, ITS2-MCA offers a simple, rapid and cost-effective method for identification of cultured isolates of the clinically most relevant and prevalent Candida species. Further studies will be necessary to evaluate how it performs on mixed samples and clinical samples.

AB - Rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. We evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA) as an identification tool for distinguishing between 16 Candida spp., i.e. Candida albicans, Candida bracarensis, Candida dubliniensis, Candida famata, Candida glabrata, Candida guilliermondii, Candida inconspicua, Candida kefyr, Candida krusei, Candida lipolytica, Candida lusitaniae, Candida nivariensis, Candida norvegensis, Candida parapsilosis, Candida tropicalis and Candida sojae, and Saccharomyces cerevisiae and one species pair, i.e. Candida metapsilosis/Candida orthopsilosis. Starting from a cultured isolate, ITS2-MCA led to differentiation of these species within 6 h. According to our findings, ITS2-MCA offers a simple, rapid and cost-effective method for identification of cultured isolates of the clinically most relevant and prevalent Candida species. Further studies will be necessary to evaluate how it performs on mixed samples and clinical samples.

UR - http://dx.doi.org/10.1016/j.resmic.2012.10.017

U2 - 10.1016/j.resmic.2012.10.017

DO - 10.1016/j.resmic.2012.10.017

M3 - Article

SN - 0923-2508

VL - 164

SP - 110

EP - 117

JO - Research in Microbiology

JF - Research in Microbiology

IS - 2

ER -

Rapid and accurate identification of isolates of Candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (ITS2-MCA)

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