Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience

Paul A De Sousa; Rachel Steeg; Elisabeth Wachter; Kevin Bruce; Jason King; Marieke Hoeve; Shalinee Khadun; George McConnachie; Julie Holder; Andreas Kurtz; Stefanie Seltmann; Johannes Dewender; Sascha Reimann; Glyn Stacey; Orla O'Shea; Charlotte Chapman; Lyn Healy; Heiko Zimmermann; Bryan Bolton; Trisha Rawat; Isobel Atkin; Anna Veiga; Bernd Kuebler; Blanca Miranda Serano; Tomo Saric; Jürgen Hescheler; Oliver Brüstle; Michael Peitz; Cornelia Thiele; Niels Geijsen; Bjørn Holst; Christian Clausen; Majlinda Lako; Lyle Armstrong; Shailesh K Gupta; Alexander J Kvist; Ryan Hicks; Anna Jonebring; Gabriella Brolén; Andreas Ebneth; Alfredo Cabrera-Socorro; Patrik Foerch; Martine Geraerts; Tina C Stummann; Shawn Harmon; Carol George; Ian Streeter; Laura Clarke; Helen Parkinson; Peter W Harrison; Adam Faulconbridge; Luca Cherubin; Tony Burdett; Cesar Trigueros; Minal J Patel; Christa Lucas; Barry Hardy; Rok Predan; Joh Dokler; Maja Brajnik; Oliver Keminer; Ole Pless; Philip Gribbon; Carsten Claussen; Annette Ringwald; Beate Kreisel; Aidan Courtney; Timothy E Allsopp

doi:10.1016/j.scr.2017.03.002

Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience

Paul A De Sousa, Rachel Steeg, Elisabeth Wachter, Kevin Bruce, Jason King, Marieke Hoeve, Shalinee Khadun, George McConnachie, Julie Holder, Andreas Kurtz, Stefanie Seltmann, Johannes Dewender, Sascha Reimann, Glyn Stacey, Orla O'Shea, Charlotte Chapman, Lyn Healy, Heiko Zimmermann, Bryan Bolton, Trisha RawatIsobel Atkin, Anna Veiga, Bernd Kuebler, Blanca Miranda Serano, Tomo Saric, Jürgen Hescheler, Oliver Brüstle, Michael Peitz, Cornelia Thiele, Niels Geijsen, Bjørn Holst, Christian Clausen, Majlinda Lako, Lyle Armstrong, Shailesh K Gupta, Alexander J Kvist, Ryan Hicks, Anna Jonebring, Gabriella Brolén, Andreas Ebneth, Alfredo Cabrera-Socorro, Patrik Foerch, Martine Geraerts, Tina C Stummann, Shawn Harmon, Carol George, Ian Streeter, Laura Clarke, Helen Parkinson, Peter W Harrison, Adam Faulconbridge, Luca Cherubin, Tony Burdett, Cesar Trigueros, Minal J Patel, Christa Lucas, Barry Hardy, Rok Predan, Joh Dokler, Maja Brajnik, Oliver Keminer, Ole Pless, Philip Gribbon, Carsten Claussen, Annette Ringwald, Beate Kreisel, Aidan Courtney, Timothy E Allsopp

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

53 Citations (Scopus)

Abstract

A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.

Original language	English
Pages (from-to)	105-114
Number of pages	10
Journal	Stem Cell Research
Volume	20
DOIs	https://doi.org/10.1016/j.scr.2017.03.002
Publication status	Published - Apr 2017

Keywords

Biological Specimen Banks
Cell Line
Cryopreservation
Europe
Humans
Induced Pluripotent Stem Cells
Journal Article

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10.1016/j.scr.2017.03.002

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De Sousa, P. A., Steeg, R., Wachter, E., Bruce, K., King, J., Hoeve, M., Khadun, S., McConnachie, G., Holder, J., Kurtz, A., Seltmann, S., Dewender, J., Reimann, S., Stacey, G., O'Shea, O., Chapman, C., Healy, L., Zimmermann, H., Bolton, B., ... Allsopp, T. E. (2017). Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience. Stem Cell Research, 20, 105-114. https://doi.org/10.1016/j.scr.2017.03.002

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title = "Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience",

abstract = "A fast track {"}Hot Start{"} process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.",

keywords = "Biological Specimen Banks, Cell Line, Cryopreservation, Europe, Humans, Induced Pluripotent Stem Cells, Journal Article",

author = "{De Sousa}, {Paul A} and Rachel Steeg and Elisabeth Wachter and Kevin Bruce and Jason King and Marieke Hoeve and Shalinee Khadun and George McConnachie and Julie Holder and Andreas Kurtz and Stefanie Seltmann and Johannes Dewender and Sascha Reimann and Glyn Stacey and Orla O'Shea and Charlotte Chapman and Lyn Healy and Heiko Zimmermann and Bryan Bolton and Trisha Rawat and Isobel Atkin and Anna Veiga and Bernd Kuebler and Serano, {Blanca Miranda} and Tomo Saric and J{\"u}rgen Hescheler and Oliver Br{\"u}stle and Michael Peitz and Cornelia Thiele and Niels Geijsen and Bj{\o}rn Holst and Christian Clausen and Majlinda Lako and Lyle Armstrong and Gupta, {Shailesh K} and Kvist, {Alexander J} and Ryan Hicks and Anna Jonebring and Gabriella Brol{\'e}n and Andreas Ebneth and Alfredo Cabrera-Socorro and Patrik Foerch and Martine Geraerts and Stummann, {Tina C} and Shawn Harmon and Carol George and Ian Streeter and Laura Clarke and Helen Parkinson and Harrison, {Peter W} and Adam Faulconbridge and Luca Cherubin and Tony Burdett and Cesar Trigueros and Patel, {Minal J} and Christa Lucas and Barry Hardy and Rok Predan and Joh Dokler and Maja Brajnik and Oliver Keminer and Ole Pless and Philip Gribbon and Carsten Claussen and Annette Ringwald and Beate Kreisel and Aidan Courtney and Allsopp, {Timothy E}",

year = "2017",

month = apr,

doi = "10.1016/j.scr.2017.03.002",

language = "English",

volume = "20",

pages = "105--114",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier B.V.",

}

De Sousa, PA, Steeg, R, Wachter, E, Bruce, K, King, J, Hoeve, M, Khadun, S, McConnachie, G, Holder, J, Kurtz, A, Seltmann, S, Dewender, J, Reimann, S, Stacey, G, O'Shea, O, Chapman, C, Healy, L, Zimmermann, H, Bolton, B, Rawat, T, Atkin, I, Veiga, A, Kuebler, B, Serano, BM, Saric, T, Hescheler, J, Brüstle, O, Peitz, M, Thiele, C, Geijsen, N, Holst, B, Clausen, C, Lako, M, Armstrong, L, Gupta, SK, Kvist, AJ, Hicks, R, Jonebring, A, Brolén, G, Ebneth, A, Cabrera-Socorro, A, Foerch, P, Geraerts, M, Stummann, TC, Harmon, S, George, C, Streeter, I, Clarke, L, Parkinson, H, Harrison, PW, Faulconbridge, A, Cherubin, L, Burdett, T, Trigueros, C, Patel, MJ, Lucas, C, Hardy, B, Predan, R, Dokler, J, Brajnik, M, Keminer, O, Pless, O, Gribbon, P, Claussen, C, Ringwald, A, Kreisel, B, Courtney, A & Allsopp, TE 2017, 'Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience', Stem Cell Research, vol. 20, pp. 105-114. https://doi.org/10.1016/j.scr.2017.03.002

TY - JOUR

T1 - Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience

AU - De Sousa, Paul A

AU - Steeg, Rachel

AU - Wachter, Elisabeth

AU - Bruce, Kevin

AU - King, Jason

AU - Hoeve, Marieke

AU - Khadun, Shalinee

AU - McConnachie, George

AU - Holder, Julie

AU - Kurtz, Andreas

AU - Seltmann, Stefanie

AU - Dewender, Johannes

AU - Reimann, Sascha

AU - Stacey, Glyn

AU - O'Shea, Orla

AU - Chapman, Charlotte

AU - Healy, Lyn

AU - Zimmermann, Heiko

AU - Bolton, Bryan

AU - Rawat, Trisha

AU - Atkin, Isobel

AU - Veiga, Anna

AU - Kuebler, Bernd

AU - Serano, Blanca Miranda

AU - Saric, Tomo

AU - Hescheler, Jürgen

AU - Brüstle, Oliver

AU - Peitz, Michael

AU - Thiele, Cornelia

AU - Geijsen, Niels

AU - Holst, Bjørn

AU - Clausen, Christian

AU - Lako, Majlinda

AU - Armstrong, Lyle

AU - Gupta, Shailesh K

AU - Kvist, Alexander J

AU - Hicks, Ryan

AU - Jonebring, Anna

AU - Brolén, Gabriella

AU - Ebneth, Andreas

AU - Cabrera-Socorro, Alfredo

AU - Foerch, Patrik

AU - Geraerts, Martine

AU - Stummann, Tina C

AU - Harmon, Shawn

AU - George, Carol

AU - Streeter, Ian

AU - Clarke, Laura

AU - Parkinson, Helen

AU - Harrison, Peter W

AU - Faulconbridge, Adam

AU - Cherubin, Luca

AU - Burdett, Tony

AU - Trigueros, Cesar

AU - Patel, Minal J

AU - Lucas, Christa

AU - Hardy, Barry

AU - Predan, Rok

AU - Dokler, Joh

AU - Brajnik, Maja

AU - Keminer, Oliver

AU - Pless, Ole

AU - Gribbon, Philip

AU - Claussen, Carsten

AU - Ringwald, Annette

AU - Kreisel, Beate

AU - Courtney, Aidan

AU - Allsopp, Timothy E

PY - 2017/4

Y1 - 2017/4

N2 - A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.

AB - A fast track "Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement, bio-sample tracking, iPSC expansion, cryopreservation, qualification and distribution to the research community. These were implemented to create a quality managed foundational collection of lines and associated data made available for distribution. Here we report on the successful outcome of this experience and work flow for banking and facilitating access to an otherwise disparate European resource, with lessons to benefit the international research community. ETOC: The report focuses on the EBiSC experience of rapidly establishing an operational capacity to procure, bank and distribute a foundational collection of established hiPSC lines. It validates the feasibility and defines the challenges of harnessing and integrating the capability and productivity of centres across Europe using commonly available resources currently in the field.

KW - Biological Specimen Banks

KW - Cell Line

KW - Cryopreservation

KW - Europe

KW - Humans

KW - Induced Pluripotent Stem Cells

KW - Journal Article

U2 - 10.1016/j.scr.2017.03.002

DO - 10.1016/j.scr.2017.03.002

M3 - Article

C2 - 28334554

SN - 1873-5061

VL - 20

SP - 105

EP - 114

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience

Abstract

Keywords

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