Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS

Abdullah Ms Al-Hatmi, Anne-Cécile Normand, Anne D van Diepeningen, Marijke Hendrickx, G Sybren de Hoog, Renaud Piarroux

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    29 Citations (Scopus)

    Abstract

    AIM: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories.

    MATERIALS & METHODS: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard.

    RESULTS: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of ≥ 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra.

    CONCLUSION: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.

    Original languageEnglish
    Pages (from-to)1939-52
    Number of pages14
    JournalFuture Microbiology
    Volume10
    DOIs
    Publication statusPublished - Dec 2015

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