Rapid screening for genotypes as possible markers of virulence in the neurotropic black yeast Exophiala dermatiddis using PCR-RFLP

M. Sudhadham, G.S. de Hoog, S.B.J. Menken, A. H. Gerrits Van Den Ende, P. Sihanonth

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    15 Citations (Scopus)

    Abstract

    A simple method for fungal genotype screening was developed for the black yeast Exophiala dermatitidis based on RFLP of ribosomal ITS regions currently used as potential virulence markers. In a study set of 502 strains of the species, two main genotypes were recognized. Only 0.97% of lanes were difficult to interpret as they did not clearly present one of the expected genotypes. Twenty strains were deviating and proved to be E. spinifera after sequencing. Eight common. related species (based on SSU data) with clinical significance yielded different patterns with TaqI digestion, and thus the method is also usable for routine diagnostics. (C) 2009 Published by Elsevier B.V.
    Original languageEnglish
    Pages (from-to)138-142
    JournalJournal of Microbiological Methods
    Volume80
    Issue number2
    DOIs
    Publication statusPublished - 2010

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