Rapid screening for human-pathogenic Mucorales using rolling circle amplification

S Dolatabadi; M J Najafzadeh; G S de Hoog

doi:10.1111/myc.12245

Rapid screening for human-pathogenic Mucorales using rolling circle amplification

S Dolatabadi, M J Najafzadeh, G S de Hoog

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

20 Citations (Scopus)

Abstract

Mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. Source of transmission is from unidentified sources in the environment. Early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. In this study, rolling circle amplification (RCA) is introduced as a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of six of the most virulent species (Rhizopus microsporus, R. arrhizus var. arrhizus, R. arrhizus var. delemar, Mucor irregularis, Mucor circinelloides, Lichtheimia ramosa, Lichtheimia corymbifera). DNAs of target species were successfully amplified, with no cross reactivity between species. RCA can be considered as a rapid detection method with high specificity and sensitivity, suitable for large screening.

Original language	English
Pages (from-to)	67-72
Number of pages	6
Journal	Mycoses
Volume	57 Suppl 3
DOIs	https://doi.org/10.1111/myc.12245
Publication status	Published - Dec 2014

Keywords

Mucorales
mucormycosis
environmental screening
rolling circle amplification

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10.1111/myc.12245

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Dolatabadi_et_al-2014-Mycoses
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title = "Rapid screening for human-pathogenic Mucorales using rolling circle amplification",

abstract = "Mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. Source of transmission is from unidentified sources in the environment. Early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. In this study, rolling circle amplification (RCA) is introduced as a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of six of the most virulent species (Rhizopus microsporus, R. arrhizus var. arrhizus, R. arrhizus var. delemar, Mucor irregularis, Mucor circinelloides, Lichtheimia ramosa, Lichtheimia corymbifera). DNAs of target species were successfully amplified, with no cross reactivity between species. RCA can be considered as a rapid detection method with high specificity and sensitivity, suitable for large screening.",

keywords = "Mucorales, mucormycosis, environmental screening, rolling circle amplification",

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year = "2014",

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doi = "10.1111/myc.12245",

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volume = "57 Suppl 3",

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AU - Dolatabadi, S

AU - Najafzadeh, M J

AU - de Hoog, G S

PY - 2014/12

Y1 - 2014/12

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AB - Mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. Source of transmission is from unidentified sources in the environment. Early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. In this study, rolling circle amplification (RCA) is introduced as a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of six of the most virulent species (Rhizopus microsporus, R. arrhizus var. arrhizus, R. arrhizus var. delemar, Mucor irregularis, Mucor circinelloides, Lichtheimia ramosa, Lichtheimia corymbifera). DNAs of target species were successfully amplified, with no cross reactivity between species. RCA can be considered as a rapid detection method with high specificity and sensitivity, suitable for large screening.

KW - Mucorales

KW - mucormycosis

KW - environmental screening

KW - rolling circle amplification

U2 - 10.1111/myc.12245

DO - 10.1111/myc.12245

M3 - Article

C2 - 25187214

SN - 0933-7407

VL - 57 Suppl 3

SP - 67

EP - 72

JO - Mycoses

JF - Mycoses

ER -

Rapid screening for human-pathogenic Mucorales using rolling circle amplification

Abstract

Keywords

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