Rapid screening for human-pathogenic Mucorales using rolling circle amplification

S Dolatabadi, M J Najafzadeh, G S de Hoog

    Research output: Contribution to journal/periodicalArticleScientificpeer-review

    19 Citations (Scopus)

    Abstract

    Mucormycosis has emerged as a relatively common severe mycosis in patients with haematological and allogeneic stem cell transplantation. Source of transmission is from unidentified sources in the environment. Early diagnosis of infection and its source of contamination are paramount for rapid and appropriate therapy. In this study, rolling circle amplification (RCA) is introduced as a sensitive, specific and reproducible isothermal DNA amplification technique for rapid molecular identification of six of the most virulent species (Rhizopus microsporus, R. arrhizus var. arrhizus, R. arrhizus var. delemar, Mucor irregularis, Mucor circinelloides, Lichtheimia ramosa, Lichtheimia corymbifera). DNAs of target species were successfully amplified, with no cross reactivity between species. RCA can be considered as a rapid detection method with high specificity and sensitivity, suitable for large screening.

    Original languageEnglish
    Pages (from-to)67-72
    Number of pages6
    JournalMycoses
    Volume57 Suppl 3
    DOIs
    Publication statusPublished - Dec 2014

    Keywords

    • Mucorales
    • mucormycosis
    • environmental screening
    • rolling circle amplification

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