RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.

F.A. Steiner; K.L. Okihara; S.W. Hoogstrate; T. Sijen; R.F. Ketting

doi:10.1038/nsmb.1541

RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.

F.A. Steiner, K.L. Okihara, S.W. Hoogstrate, T. Sijen, R.F. Ketting

Hubrecht Institute

Research output: Contribution to journal/periodical › Article › Scientific › peer-review

Abstract

RNA interference (RNAi) is a process in which double-stranded RNA is cleaved into small interfering RNAs (siRNAs) that induce the destruction of homologous single-stranded mRNAs. Argonaute proteins are essential components of this silencing process; they bind siRNAs directly and can cleave RNA targets using a conserved RNase H motif. In Caenorhabditis elegans, the Argonaute protein RDE-1 has a central role in RNAi. In animals lacking RDE-1, the introduction of double-stranded RNA does not trigger any detectable level of RNAi. Here we show that RNase H activity of RDE-1 is required only for efficient removal of the passenger strand of the siRNA duplex and not for triggering the silencing response at the target-mRNA level. These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo.

Original language	English
Pages (from-to)	207-211
Journal	Nature Structural & Molecular Biology
Volume	16
Issue number	2
DOIs	https://doi.org/10.1038/nsmb.1541
Publication status	Published - 2009

Access to Document

10.1038/nsmb.1541

Cite this

@article{e8a403539794453191ac69dc99cd3ae7,

title = "RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.",

abstract = "RNA interference (RNAi) is a process in which double-stranded RNA is cleaved into small interfering RNAs (siRNAs) that induce the destruction of homologous single-stranded mRNAs. Argonaute proteins are essential components of this silencing process; they bind siRNAs directly and can cleave RNA targets using a conserved RNase H motif. In Caenorhabditis elegans, the Argonaute protein RDE-1 has a central role in RNAi. In animals lacking RDE-1, the introduction of double-stranded RNA does not trigger any detectable level of RNAi. Here we show that RNase H activity of RDE-1 is required only for efficient removal of the passenger strand of the siRNA duplex and not for triggering the silencing response at the target-mRNA level. These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo.",

author = "F.A. Steiner and K.L. Okihara and S.W. Hoogstrate and T. Sijen and R.F. Ketting",

note = "Reporting year: 2009",

year = "2009",

doi = "10.1038/nsmb.1541",

language = "English",

volume = "16",

pages = "207--211",

journal = "Nature Structural and Molecular Biology",

issn = "1545-9993",

publisher = "Nature Publishing Group",

number = "2",

}

TY - JOUR

T1 - RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.

AU - Steiner, F.A.

AU - Okihara, K.L.

AU - Hoogstrate, S.W.

AU - Sijen, T.

AU - Ketting, R.F.

N1 - Reporting year: 2009

PY - 2009

Y1 - 2009

N2 - RNA interference (RNAi) is a process in which double-stranded RNA is cleaved into small interfering RNAs (siRNAs) that induce the destruction of homologous single-stranded mRNAs. Argonaute proteins are essential components of this silencing process; they bind siRNAs directly and can cleave RNA targets using a conserved RNase H motif. In Caenorhabditis elegans, the Argonaute protein RDE-1 has a central role in RNAi. In animals lacking RDE-1, the introduction of double-stranded RNA does not trigger any detectable level of RNAi. Here we show that RNase H activity of RDE-1 is required only for efficient removal of the passenger strand of the siRNA duplex and not for triggering the silencing response at the target-mRNA level. These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo.

AB - RNA interference (RNAi) is a process in which double-stranded RNA is cleaved into small interfering RNAs (siRNAs) that induce the destruction of homologous single-stranded mRNAs. Argonaute proteins are essential components of this silencing process; they bind siRNAs directly and can cleave RNA targets using a conserved RNase H motif. In Caenorhabditis elegans, the Argonaute protein RDE-1 has a central role in RNAi. In animals lacking RDE-1, the introduction of double-stranded RNA does not trigger any detectable level of RNAi. Here we show that RNase H activity of RDE-1 is required only for efficient removal of the passenger strand of the siRNA duplex and not for triggering the silencing response at the target-mRNA level. These results uncouple the role of the RDE-1 RNase H activity in small RNA maturation from its role in target-mRNA silencing in vivo.

U2 - 10.1038/nsmb.1541

DO - 10.1038/nsmb.1541

M3 - Article

VL - 16

SP - 207

EP - 211

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 2

ER -

RDE-1 slicer activity is required only for passenger-strand cleavage during RNAi in Caenorhabditis elegans.

Abstract

Access to Document

Fingerprint

Cite this